LOCUS       sequence-125615-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express humanized Cas9 under the control of trpC promoter and 
            terminator from A. nidulans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p415-PtrpC-Cas9-TtrpC-CYC1t
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..41
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        23..41
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        55..412
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
ORIGIN
        1 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagaaga
       61 tgatattgaa ggagcatttt tgggcttggc tggagctagt ggaggtcaac aatgaatgcc
      121 tattttggtt tagtcgtcca ggcggtgagc acaaaatttg tgtcgtttga caagatggtt
      181 catttaggca actggtcaga tcatccccac ttgtagcagt agcggcggcg ctcgaagtgt
      241 gactcttatt agcagacagg aacgaggaca ttattatcat ctgctgcttg gtgcacgata
      301 acttggtgcg tttgtcaagc aaggtaagtg aacgacccgg tcataccttc ttaagttcgc
      361 ccttcctccc tttatttcag attcaatctg acttacctat tctaccgaag cactagtgga
      421 tcccccggga aaaaaatgga caagaagtac tccattgggc tcgatatcgg cacaaacagc
      481 gtcggttggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg
      541 ggcaataccg atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc
      601 ggggagacgg ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga
      661 aagaatcgga tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac
      721 tctttcttcc ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc
      781 cacccaatct ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata
      841 tatcatctga ggaagaagct tgtagacagt actgataagg ctgacttgc
//
