LOCUS       sequence-125617-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized CMtCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY012 (pcDNA3.1-hCMtCpf1)
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgaacaat tacgacgagt
      121 tcaccaagct gtatcctatc cagaaaacca tccggtttga gctgaagcca cagggcagaa
      181 ccatggagca cctggagaca ttcaacttct ttgaggagga ccgggataga gccgagaagt
      241 ataagatcct gaaggaggcc atcgacgagt accacaagaa gtttatcgat gagcacctga
      301 ccaatatgtc cctggattgg aactctctga agcagatcag cgagaagtac tataagagca
      361 gggaggagaa ggacaagaag gtgttcctgt ccgagcagaa gaggatgcgc caggagatcg
      421 tgtctgagtt taagaaggac gatcgcttca aggacctgtt ttccaagaag ctgttctctg
      481 agctgctgaa ggaggagatc tacaagaagg gcaaccacca ggagatcgac gccctgaaga
      541 gcttcgataa gttttccggc tatttcatcg gcctgcacga gaataggaag aacatgtact
      601 ccgacggcga tgagatcacc gccatctcca atcgcatcgt gaatgagaac ttccccaagt
      661 ttctggataa cctgcagaag taccaggagg ccaggaagaa gtatcctgag tggatcatca
      721 aggccgagag cgccctggtg gcccacaata tcaagatgga cgaggtgttc tccctggagt
      781 actttaataa ggtgctgaac caggagggca tccagcggta caacctggcc ctgggcggct
      841 atgtgaccaa gagcggcgag aagatgatgg gcctgaatga tgccctgaac ctgg
//
