LOCUS       sequence-125619-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized CMtCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY012 (pcDNA3.1-hCMtCpf1)
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..130)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(137..184)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg
       61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat
      121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      181 ttttgtcgcc tctggtctgc ataaactcga accaatcctt gtgcttcagc tccagcttgg
      241 ccatcttctc gctatcaggg tcgaacttct ctgcgattgc cctcattgtc agctctcccc
      301 tcagggcgat gttgtaggcg ccattggcat cggcgtcgat aggcagctcg cgcctcttgg
      361 ggtcggtcct aaagaactcg cccttggagt tcttgatggg gctgatgata taatcctcct
      421 tgccgtcgta cacgcgcatc tggatggcgg cgatgaagct agaatacatt gtgtagatca
      481 ggccgttatt gttgctcctc aggatgtctg gcaggatgtt ctggccgcca tcgtacttga
      541 tgccggagct ggtcagggcc tccttgatct ccttagaagg gtcaaacagc tcattccgct
      601 tcttctcctt gatgtagcgc atcctctcgc cgtttgtata ggcggtccac acgttcttgt
      661 gatctgtctt gctggtgccg aactttctgt agtcaaaggc gaaggcaaag atgccgccat
      721 ccttggcaga ataggagatg ctctcaaact tctgcaggaa ctccttccgc tcctgggcgt
      781 ttgtcttaga ggaggtgtta aacagattca cgaagcctgt ggtggggtcg atcttggatg
      841 tataggcggc tggcacgtaa aacaggatgc cggtctgctt gcccagcttg gcgaaagact
      901 ccagtgggtt tgt
//
