LOCUS       sequence-125620-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral GFP reporter plasmid for high throughput functional 
            detection of active transcriptional regulatory DNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Murtha M, Tokcaer-Keskin Z, Tang Z, Strino F, Chen X, Wang Y, Xi X, 
            Basilico C, Brown S, Bonneau R, Kluger Y, Dailey L
  TITLE     FIREWACh: high-throughput functional detection of transcriptional 
            regulatory modules in mammalian cells.
  JOURNAL   Nat Methods. 2014 May;11(5):559-65. doi: 10.1038/nmeth.2885. Epub 
            2014 Mar 23.
  PUBMED    24658142
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2885"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "559-65"
COMMENT     Sequence Label: FpG5
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    147..228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(184..203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        257..656
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
       61 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
      121 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
      181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgctt aattaacccg
      241 tgtcggctcc agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
      301 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
      361 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
      421 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
      481 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
      541 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
      601 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
      661 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
      721 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
      781 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
      841 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
      901 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
      961 accatctggg gaccctgacg tgaagtttg
//
