LOCUS       sequence-125622-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato in pTREX-b vector which confers resistance to 
            blasticidin. This vector is used for cloning a specific sgRNA by 
            BamHI, to transfect Cas9-expressing Trypanosoma cruzi 
            epimastigotes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Lander N, Li ZH, Niyogi S, Docampo R
  TITLE     CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 
            Genes in Trypanosoma cruzi Reveals Their Role in Flagellar 
            Attachment.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15.
  PUBMED    26199333
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e01012-15. doi"; pages: " 
            10.1128/mBio.01012-15"
COMMENT     Sequence Label: tdTomato/pTREX-b
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(76..99)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(274..293)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     543..561
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 actcgtccat gccgtacagg aacaggtggt ggcggccctc ggagcgctcg tactgttcca
       61 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg tagtagtagc
      121 cgggcagttg cacgggcttc ttggccatgt agatggtctt gaactccacc aggtagtggc
      181 cgccgtcctt cagcttcagg gcctggtgga tctcgccctt cagcacgccg tcgcgggggt
      241 acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
      301 gggggaagtt ggtgccgcgc atcttcacct tgtagatcag cgtgccgtcc tgcagggagg
      361 agtcctgggt cacggtcacc agaccgccgt cctcgaagtt catcacgcgc tcccacttga
      421 agccctcggg gaaggacagc ttcttgtaat cggggatgtc ggcggggtgc ttcacgtacg
      481 ccttggagcc gtacatgaac tggggggaca ggatgtccca ggcgaagggc agggggccgc
      541 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
      601 cctcgatctc gaactcgtgg ccgttcatgg agccctccat gcgcaccttg aagcgcatga
      661 actctttgat gacggccatg ttgttgtcct cggaggaggc ggtgccggag ctgccgctgc
      721 cggtgctgcc ggtgccatgc ccca
//
