LOCUS       sequence-125625-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized EeCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY013 (pcDNA3.1-hEeCpf1)
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgaacggc aataggtcca
      121 tcgtgtaccg cgagttcgtg ggcgtgatcc ccgtggccaa gaccctgagg aatgagctgc
      181 gccctgtggg ccacacacag gagcacatca tccagaacgg cctgatccag gaggacgagc
      241 tgcggcagga gaagagcacc gagctgaaga acatcatgga cgattactat agagagtaca
      301 tcgataagtc tctgagcggc gtgaccgacc tggacttcac cctgctgttc gagctgatga
      361 acctggtgca gagctccccc tccaaggaca ataagaaggc cctggagaag gagcagtcta
      421 agatgaggga gcagatctgc acccacctgc agtccgactc taactacaag aatatcttta
      481 acgccaagct gctgaaggag atcctgcctg atttcatcaa gaactacaat cagtatgacg
      541 tgaaggataa ggccggcaag ctggagacac tggccctgtt taatggcttc agcacatact
      601 ttaccgactt ctttgagaag aggaagaacg tgttcaccaa ggaggccgtg agcacatcca
      661 tcgcctaccg catcgtgcac gagaactccc tgatcttcct ggccaatatg acctcttata
      721 agaagatcag cgagaaggcc ctggatgaga tcgaagtgat cgagaagaac aatcaggaca
      781 agatgggcga ttgggagctg aatcagatct ttaaccctga cttctacaat atggtgctga
      841 tccagtccgg catcgacttc tacaacgaga tctgcggcgt ggtgaatgcc cacatgaacc
      901 tgtactgtca gca
//
