LOCUS       sequence-125626-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato in pTREX-b vector which confers resistance to 
            blasticidin. This vector is used for cloning a specific sgRNA by 
            BamHI, to transfect Cas9-expressing Trypanosoma cruzi 
            epimastigotes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Lander N, Li ZH, Niyogi S, Docampo R
  TITLE     CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 
            Genes in Trypanosoma cruzi Reveals Their Role in Flagellar 
            Attachment.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15.
  PUBMED    26199333
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e01012-15. doi"; pages: " 
            10.1128/mBio.01012-15"
COMMENT     Sequence Label: tdTomato/pTREX-b
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(98..121)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(296..315)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     565..583
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ccggagctgc cgctgccggt gctgccggtg ccatgcccca ggaacaggtg gtggcggccc
       61 tcggagcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc
      121 ttggtgtcca cgtagtagta gccgggcagt tgcacgggct tcttggccat gtagatggtc
      181 ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca gggcctggtg gatctcgccc
      241 ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc catggtcttc
      301 ttctgcatta cggggccgtc gggggggaag ttggtgccgc gcatcttcac cttgtagatc
      361 agcgtgccgt cctgcaggga ggagtcctgg gtcacggtca ccagaccgcc gtcctcgaag
      421 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcttgta atcggggatg
      481 tcggcggggt gcttcacgta cgccttggag ccgtacatga actgggggga caggatgtcc
      541 caggcgaagg gtagggggcc gcccttggtc accttcagct tggcggtctg ggtgccttcg
      601 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcat ggagccctcc
      661 atgcgcacct tgaagcgcat gaactctttg atgacctcct cacccttgga aaccattcta
      721 gacaagacaa cttatagagc aaaaaaaaa
//
