LOCUS       sequence-125627-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for homologous recombination to insert a 2A-mCherry-NLS
            cassette in place of the stop codon of myogenin in the mouse 
            genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Gersbach
  TITLE     TMG reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Myog'-2A-mCherryNLS-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..167)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat tggcccggga
      121 ttctcttcga catcccctgc ttgtttcaac agggagaagt tagtggcgct agcgttgggc
      181 atggtttcgt ctgggaaggc aacagacata tcctccaccg tgatgctgtc gacgatggac
      241 gtaagggagt gcagattgtg ggcgtctgta gggtcagccg cgagcaaatg atctgcaaga
      301 aaaagtggga gaatgtttcc tgggcaactg gtatgggacc cttgacacag attttccagg
      361 acaaaatggg taaggaagac tcaggagtgt tccccgcccc caggacacta gtactcccca
      421 tgctgtgtgt gtgcttgtgg ggttttaaaa gtggctatcc ctaggtgaag ccaggatatc
      481 ctagggcaca gggcacagtt ttacaacact gactgagtga tccccaggga cccctggggc
      541 aggagcaggc tcttctctct gaggtcagag accagcaggg aagttgctgg aggctctgac
      601 tactctttag ctggtcaagg ctaccgggag gcacagggtc tccagcccac catttggggc
      661 cctcaggtca gcaagttcct agaaaagctg ctgaagggtg attgggacag ggcttatggt
      721 aagggtacaa gcccagccta ggtagggact aagggatttg agccatgtta ccagggtcag
      781 gattcactta cctcctgggt tgggaccgaa ctccagtgca ttgccccact ccggactgca
      841 ggaggcgctg
//
