LOCUS       sequence-125628-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized MbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY014 (pcDNA3.1-hMbCpf1)
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgctgttc caggacttta
      121 cccacctgta tccactgtcc aagacagtga gatttgagct gaagcccatc gataggaccc
      181 tggagcacat ccacgccaag aacttcctgt ctcaggacga gacaatggcc gatatgcacc
      241 agaaggtgaa agtgatcctg gacgattacc accgcgactt catcgccgat atgatgggcg
      301 aggtgaagct gaccaagctg gccgagttct atgacgtgta cctgaagttt cggaagaacc
      361 caaaggacga tgagctgcag aagcagctga aggatctgca ggccgtgctg agaaaggaga
      421 tcgtgaagcc catcggcaat ggcggcaagt ataaggccgg ctacgacagg ctgttcggcg
      481 ccaagctgtt taaggacggc aaggagctgg gcgatctggc caagttcgtg atcgcacagg
      541 agggagagag ctccccaaag ctggcccacc tggcccactt cgagaagttt tccacctatt
      601 tcacaggctt tcacgataac cggaagaata tgtattctga cgaggataag cacaccgcca
      661 tcgcctaccg cctgatccac gagaacctgc cccggtttat cgacaatctg cagatcctga
      721 ccacaatcaa gcagaagcac tctgccctgt acgatcagat catcaacgag ctgaccgcca
      781 gcggcctgga cgtgtctctg gccagccacc tggatggcta tcacaagctg ctgacacagg
      841 agggcatcac cgcctacaat acactgctgg gaggaatctc cggagaggca ggctctccta
      901 agatccaggg catcaacgag ctgat
//
