LOCUS       sequence-125629-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for homologous recombination to insert a 2A-mCherry-NLS
            cassette in place of the stop codon of myogenin in the mouse 
            genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Gersbach
  TITLE     TMG reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Myog'-2A-mCherryNLS-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    585..618
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
       61 aaggagcgca cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga
      121 tctattaaac aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag
      181 aagggtgaga acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg
      241 tggggtggga ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga
      301 taatgtttca tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc
      361 attcctccca ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct
      421 tgattcccac tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg
      481 aagaacgaga tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa
      541 gttctaattc catcagaagc tggtcgagat ccggaaccct taatataact tcgtataatg
      601 tatgctatac gaagttatta ggtccctcga agaggttcac taggcgcgcc gattgtctgt
      661 caggctgggt gtgcatgtga gccccc
//
