LOCUS       sequence-125633-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato in pTREX-b vector which confers resistance to 
            blasticidin. This vector is used for cloning a specific sgRNA by 
            BamHI, to transfect Cas9-expressing Trypanosoma cruzi 
            epimastigotes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Lander N, Li ZH, Niyogi S, Docampo R
  TITLE     CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 
            Genes in Trypanosoma cruzi Reveals Their Role in Flagellar 
            Attachment.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15.
  PUBMED    26199333
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e01012-15. doi"; pages: " 
            10.1128/mBio.01012-15"
COMMENT     Sequence Label: tdTomato/pTREX-b
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg agctcggtgc accctgggat atttgcgcac
       61 ccacctttcc cgtcgcaatc ggcgccgtcc gaacgcggaa atgttccgag aataatacgg
      121 ctgggctgcg ccgcgcgttc cccgcgtgaa ccaactattg tgcgatgtgc agcatcctat
      181 tgtggtgctt taccgcacac cagtttcctt gcggggctta tttggtgtgt tggattttta
      241 attttttgat gattcggagg aattttttgc aatttttggg tacccctaaa aaaattttgg
      301 agtttccggg gttcgatcca acttgtgctt ttttaaccgt ccatttgtgt ctagtacatc
      361 atggagcggt attctctccg gtggcccagg tggcgtcgga aggaatcggt gtccctcagg
      421 atgtttggtt tttcgttttt ttttttgcgg tcttttgttt cgcatcgccg tatcgtgtgc
      481 ggcgcgacct tctttttggc tcattggtca gtgaggttta taataaagtg gatcatttgg
      541 gtttttggcc ctttgcggcg cacccgcgga atgggggagg gattttcata taattgtagt
      601 gtacacattg tgtgtgggtc gcaaaatgtc atgccgtgtg caatgtttat ttgcgtgctg
      661 tgcgtgtcct tttgtgattc caccacttgt gtgtatttta ccttatcgtc tctccctgcc
      721 gttggctgtg cgtgaattgt gcttcgcatt cgtgggaaat caaaagggga atcaacgtgt
      781 tctttttttt tcagaagggg agtcaaagcg ccgtgtggat gccaagtctg tgcaagagtt
      841 ctagtggatc ccccaacgag tttcttcaaa atatgcagcg gattcactaa gaaacatttt
      901 cacgcacgaa agcgaaatta ttatgattgt tatta
//
