LOCUS       sequence-125634-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses guide RNA targeting near the stop codon of myogenin in the
            mouse genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Gersbach
  TITLE     TMG reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZDonor-U6-myogenin stop codon gRNA
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(28..103)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(124..372)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(352..372)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(386..405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(387..405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(415..432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(415..431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(424..446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(660..679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aagctggagc tccaccgcgg aaaaaaagca ccgactcggt gccacttttt caagttgata
       61 acggactagc cttattttaa cttgctattt ctagctctaa aaccgatgga cgtaagggag
      121 tgcggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt
      181 tacgataagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
      241 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
      301 aattccaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
      361 aaataggccc tcggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
      421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      481 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      541 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      601 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      661 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      721 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      781 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      841 tttgacgttg gagtccacgt tctttaatag tggactctt
//
