LOCUS       sequence-125635-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized LiCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY015 (pcDNA3.1-hLiCpf1)
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..130)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(137..184)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg
       61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat
      121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      181 ttttgcggtt cctctcccac acgaactcca gccagtcctt attcttgatc ttccactttg
      241 gtctgctcag gttctcctcc tttgtctcat tcagcaccag caggttcatc aggcccttca
      301 gggcgatgtg gtaggcgcca ttggcatcgg cgtccttggg ctcatcgtcg ctggcctcca
      361 gagagttaaa gaaccggccc ttggaatcca ccactgggct caggatgaag tccttctcct
      421 cctcgccctt cttgccattg ttctggcgca gggacagtgt ggtcttgatg taaaacagca
      481 ggctcttaaa gaacacggcg tcattcttcc tcaggatctc tggcttcaga tcctggccgt
      541 tgctgaaagg gatgtccaca aacagctcct tcagcttctc ggtgatctgg atgctattgt
      601 actggatgga gctgttggcg gtcttgctgg tgaagtaccg ctccacattt gtggtgcaga
      661 tgacccacac ccgattcttg cccagcatca ggttctcgga aaacttgcgt gtatcggcgg
      721 tgaactcaaa ccagtccatc ttagaattga acctgatgga atcaaacttg ttgatccact
      781 gcttggcctt ctcgatattc tcgtaggcag ggtgcaggaa atcgataaag cctgttctgg
      841 ggtcgatctt ggaggtgttc cagcttggca cgtagaacag aaagccggtc tgcttgctca
      901 gcttctcgaa agactgaaac tcgtctgtca gctgataggc cttcagcacg cctcctggct
      961 cggttggctt attctccttg aacaccagaa agttcagctt atcgatcagc atc
//
