LOCUS       sequence-125637-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-FAK-5
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..743
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagacca
       61 caatgggcgt aatcaagccc gacatgaaga tcaagctgaa gatggagggc aacgtgaatg
      121 gccacgcctt cgtgatcgag ggcgagggcg agggcaagcc ctacgacggc accaacacca
      181 tcaacctgga ggtgaaggag ggagcccccc tgcccttctc ctacgacatt ctgaccaccg
      241 cgttcgccta cggcaacagg gccttcacca agtaccccga cgacatcccc aactacttca
      301 agcagtcctt ccccgagggc tactcttggg agcgcaccat gaccttcgag gacaagggca
      361 tcgtgaaggt gaagtccgac atctccatgg aggaggactc cttcatctac gagatacacc
      421 tcaagggcga gaacttcccc cccaacggcc ccgtgatgca gaagaagacc accggctggg
      481 acgcctccac cgagaggatg tacgtgcgcg acggcgtgct gaagggcgac gtcaagcaca
      541 agctgctgct ggagggcggc ggccaccacc gcgttgactt caagaccatc tacagggcca
      601 agaaggcggt gaagctgccc gactatcact ttgtggacca ccgcatcgag atcctgaacc
      661 acgacaagga ctacaacaag gtgaccgttt acgagagcgc cgtggcccgc aactccaccg
      721 acggcatgga cgagctgtac aagtccggac tcagatctat ggcagcagct taccttgatc
      781 caaacttgaa tcatacacca agttcaagtg c
//
