LOCUS       sequence-125638-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato in pTREX-b vector which confers resistance to 
            blasticidin. This vector is used for cloning a specific sgRNA by 
            BamHI, to transfect Cas9-expressing Trypanosoma cruzi 
            epimastigotes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Lander N, Li ZH, Niyogi S, Docampo R
  TITLE     CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 
            Genes in Trypanosoma cruzi Reveals Their Role in Flagellar 
            Attachment.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15.
  PUBMED    26199333
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e01012-15. doi"; pages: " 
            10.1128/mBio.01012-15"
COMMENT     Sequence Label: tdTomato/pTREX-b
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..36
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt acccacacgg
       61 ctagcatact ctagcggcat atacacaggt gggctataat gcccagtatt gttcgaaaaa
      121 gtccaaagaa caacaacact acgacgcata atgcacaaac actaaactac accttaaaca
      181 ccatcagtga agaaaaagaa aaaaaaagaa aaacagctta aatccttaaa aaaaaaaaat
      241 cacactaaat taaaacagtc agttataaac aaaaagaaac tgaacaacca aaatcttcca
      301 ataacgcaaa gttgtaaaaa attaccacaa gctgcatcat acacccaatc aaatcatcta
      361 cattttcctt gcaatacccc aaatcctcgt acattgatta aacattattt cactcatcct
      421 tccgcatcat taaattgctg ctttttgttt cccaaagatg accataaata taatttcaaa
      481 aaaaaaaaag aaaaaaaagt tatatatttt tttgttgttg tccttaaagg gaaaagtaaa
      541 attgttttgc tcttgacact gtgtcacttg gatcgtggca gcaccggtaa cgatcatcac
      601 gggacacgca tcgtgtatta taagatccct attcgcaaaa gaaagacctg aagtcatcgc
      661 cacgcctaca accttgccga acgatccttc gaggccatgt ggcgtacaag gtccaccacg
      721 cggtgggagt atccccactc gttgtcgtac cacgacacaa tcttgaagaa gcggcgctcc
      781 ttcggcggag atcaattctt agccctccca cacataacca gagggcagca attcacgaat
      841 cccaactgcc gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag
      901 atcgagaagc acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc
      961 gatcgcgacg atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac
     1021 g
//
