LOCUS       sequence-125657-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized LiCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY015 (pcDNA3.1-hLiCpf1)
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catggaggac tattccggct
      121 ttgtgaacat ctactctatc cagaaaaccc tgaggttcga gctgaagcca gtgggcaaga
      181 cactggagca catcgagaag aagggcttcc tgaagaagga caagatccgg gccgaggatt
      241 acaaggccgt gaagaagatc atcgataagt accacagagc ctatatcgag gaggtgtttg
      301 attccgtgct gcaccagaag aagaagaagg acaagacccg cttttctaca cagttcatca
      361 aggagatcaa ggagttcagc gagctgtact ataagaccga gaagaacatc cccgacaagg
      421 agaggctgga ggccctgagc gagaagctgc gcaagatgct ggtgggcgcc tttaagggcg
      481 agttctccga ggaggtggcc gagaagtata agaacctgtt ttctaaggag ctgatcagga
      541 atgagatcga gaagttctgc gagacagacg aggagcgcaa gcaggtgtct aacttcaaga
      601 gcttcaccac atactttacc ggcttccact ccaacaggca gaatatctat tccgacgaga
      661 agaagtctac agccatcggc taccgcatca tccaccagaa cctgcctaag ttcctggata
      721 atctgaagat catcgagtcc atccagcggc ggttcaagga cttcccatgg tctgatctga
      781 agaagaacct gaagaagatc gataagaata tcaagctgac cgagtacttc agcatcgacg
      841 gcttcgtgaa cgtgctgaat caga
//
