LOCUS       sequence-125658-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with an improved scaffold and puro/ZsG
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..760)
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     CDS             complement(767..820)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgagcgg
       61 ccgcttaggg caaggcggag ccggaggcga tggcgtgctc ggtcaggtgc cacttctggt
      121 tcttggcgtc gctgcggtcc tcgcgggtca gcttgtgctg gatgaagtgc cagtcgggca
      181 tcttgcgggg cacggacttg gccttgtaca cggtgtcgaa ctggcagcgc aagcggccac
      241 cgtccttcag cagcaggtac atgctcacgt cgcccttcaa gatgccctgc ttgggcacgg
      301 ggatgatctt ctcgcaggag ggctcccagt tgtcggtcat cttcttcatc acggggccgt
      361 cggcggggaa gttcacgccg tagaacttgg actcgtggta catgcagttt tcttcgacgc
      421 tcacggtgat gtcggcgttg cagatgcaca cggcgccgtc ctcgaacagg aaggagcggt
      481 cccaggtgta gccggcgggg caggagttct tgaagtagtc gacgatgtcc tgggggtact
      541 cggtgaacac gcggttgccg tacatgaagg cggcggacaa gatgtcctcg gcgaagggca
      601 aggggccgcc ctccaccacg cacaggttga tggcctgctt gcccttgaag gggtagccga
      661 tgccctcgcc ggtgatcacg aacttgtggc cgtccacgca gccctccatg cggtacttca
      721 tggtcatctc cttggtcagg ccgtgcttgg actgggccat gaattctggg ccaggattct
      781 cctccacgtc accgcatgtt aggagacttc ctctgccctc tcctccggac ccgccgccgg
      841 caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg ac
//
