LOCUS       sequence-125662-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PcCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY017 (pcDNA3.1-hPcCpf1)
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catggacagc ctgaaggatt
      121 tcaccaacct gtaccccgtg tccaagacac tgcggtttga gctgaagcct gtgggcaaga
      181 ccctggagaa tatcgagaag gccggcatcc tgaaggagga tgagcacaga gccgagagct
      241 accggagagt gaagaagatc atcgatacat atcacaaggt gttcatcgac agctccctgg
      301 agaacatggc caagatgggc atcgagaatg agatcaaggc catgctgcag tccttttgcg
      361 agctgtataa gaaggaccac aggaccgagg gagaggacaa ggccctggat aagatcaggg
      421 ccgtgctgag gggcctgatc gtgggagcct tcaccggcgt gtgcggccgg cgggagaaca
      481 cagtgcagaa tgagaagtat gagagcctgt ttaaggagaa gctgatcaag gagatcctgc
      541 cagatttcgt gctgtctaca gaggccgagt ccctgccctt ttctgtggag gaggccacca
      601 gaagcctgaa ggagttcgac tcctttacat cttacttcgc cggcttttat gagaaccgga
      661 agaatatcta ctctaccaag ccccagagca cagccatcgc ctatagactg atccacgaga
      721 acctgcctaa gttcatcgat aatatcctgg tgtttcagaa gatcaaggag ccaatcgcca
      781 aggagctgga gcacatcagg gcagacttca gcgccggcgg ctacatcaag aaggatgagc
      841 gcctggagga catcttttcc ctgaactact atatccacgt gctgtctcag gccggcatcg
      901 ag
//
