LOCUS       sequence-125667-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PdCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY018 (pcDNA3.1-hPdCpf1)
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccgccacc atggagaact atcaggagtt
      121 caccaacctg tttcagctga ataagacact gagattcgag ctgaagccca tcggcaagac
      181 ctgcgagctg ctggaggagg gcaagatctt cgccagcggc tcctttctgg agaaggacaa
      241 ggtgagggcc gataacgtga gctacgtgaa gaaggagatc gacaagaagc acaagatctt
      301 tatcgaggag acactgagct ccttctctat cagcaacgat ctgctgaagc agtactttga
      361 ctgctataat gagctgaagg ccttcaagaa ggactgtaag agcgatgagg aggaggtgaa
      421 gaaaaccgcc ctgcgcaaca agtgtacctc catccagagg gccatgcgcg aggccatctc
      481 tcaggccttt ctgaagagcc cccagaagaa gctgctggcc atcaagaacc tgatcgagaa
      541 cgtgttcaag gccgacgaga atgtgcagca cttctccgag tttaccagct atttctccgg
      601 ctttgagaca aacagagaga atttctactc tgacgaggag aagtccacat ctatcgccta
      661 taggctggtg cacgataacc tgcctatctt catcaagaac atctacatct tcgagaagct
      721 gaaggagcag ttcgacgcca agaccctgag cgagatcttc gagaactaca agctgtatgt
      781 ggccggctct agcctggatg a
//
