LOCUS       sequence-125669-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PmCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY019 (pcDNA3.1-hPmCpf1)
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgaaaacc cagcacttct
      121 ttgaggactt cacaagcctg tactctctga gcaagaccat ccggtttgag ctgaagccaa
      181 tcggcaagac cctggagaac atcaagaaga atggcctgat ccggagagat gagcagagac
      241 tggacgatta cgagaagctg aagaaagtga tcgacgagta tcacgaggat ttcatcgcca
      301 acatcctgag ctccttttcc ttctctgagg agatcctgca gtcctacatc cagaatctga
      361 gcgagtccga ggccagggcc aagatcgaga aaaccatgcg cgacacactg gccaaggcct
      421 tctctgagga tgagaggtac aagagcatct ttaagaagga gctggtgaag aaggacatcc
      481 ccgtgtggtg ccctgcctat aagagcctgt gcaagaagtt cgataacttt accacatctc
      541 tggtgccctt ccacgagaac aggaagaacc tgtataccag caatgagatc acagcctcta
      601 tcccttatcg catcgtgcac gtgaacctgc caaagtttat ccagaatatc gaggccctgt
      661 gcgagctgca gaagaagatg ggcgccgacc tgtacctgga gatgatggag aacctgcgca
      721 acgtgtggcc cagcttcgtg aaaaccccag acgacctgtg caacctgaaa acctataatc
      781 acctgatggt gcagtctagc atcagcgagt acaacaggtt tgtgggcggc tattccaccg
      841 aggacggcac aaagcaccag ggcatcaacg agtggatcaa tatctacaga cagaggaata
      901 aggagatgcg cctgcctggc ctggtgttcc tgcac
//
