LOCUS       sequence-125670-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PmCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY019 (pcDNA3.1-hPmCpf1)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..122)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(129..176)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcgagcggcc gccactgtgc tggatatctg cagaattctt aggcatagtc ggggacatca
       61 tatgggtatg cataatcagg cacgtcgtag ggataagcgt aatctggaac atcgtatggg
      121 taggatccct ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttctcc
      181 acgatgccct cctgcacata tctcagccac tgtgcccttc cgatcctgtg gatggactcg
      241 tggtcgcccc tcttgattct ctgcaccacc atcaggccct tgcgggccac attgtatgct
      301 ccgtttgcat ctgcgtccac aggcagatcc ttggcctcga tcagcctgct gtcgaactgc
      361 agattcttct cgttcagggc gggagacagg atataatcct cctcgccgtc gctgttccgg
      421 atctgcatca tcaggttaaa caggtagatc aggcgcacat agaactcctt cctatcctgg
      481 ctcaggatgg ccttcttcag gtccttctcc actctgtagc cgatattaaa ctgctcgaac
      541 agctccagaa agcacaggct caggttctcg atccgctcca ccatccactt gccagacttc
      601 ttggatttgg cgatgcgaga gccgaatgtg gtcagtgtcc acagcttcct ctgtgtctcc
      661 accctcacat caaatctgga cagatccagg tcgaacagga tattgccctt gccgtcatat
      721 ctgatggcgt taaaccgatc gaaaaacttg cgggcgtcgc ccacattggt gtagttgatt
      781 ctgcccagca gattcacgaa gcctgtgctg ggatctgtca gagaggtgtt ccatgggtcc
      841 acgaaaaaca ggatgccgct ctgggggtat ctgtgcagct cctcaaaaga gaacagtgga
//
