LOCUS       sequence-125682-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with the conventional scaffold and 
            puro/BFP markers without WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(653..706)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(805..825)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctgccacctc gtgctgctcg acgtaggtct cgttgttggc ctccttgatt ctttccagtc
       61 tgtagtccac atagtagacg ccaggcatct tgaggttctt agcgggtttc ttggatctat
      121 atgtggtctt gatgtttgcg atcagatggc tcccgcccac gagcttcagg gccatgtcgt
      181 ttctgccttc caggccgccg tcagcggggt acagcgtctc ggtgaaggcc tcccagccga
      241 gtgttttctt ctgcatcaca gggccgttgg atgtgaagtt cacccctctg atcttgacgt
      301 tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccgt
      361 cctcgtatgt ggtgactctc tcccatgtga agccctcagg gaaggactgc ttgaagaagt
      421 cggggatgcc ctgggtgtgg ttgatgaagg tcttgctgcc gtagaggaag ctagtagcca
      481 ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg
      541 tgccctcgta gggcttgcct tcgccctcgg atgtgcactt gaagtgatgg ttgtccacgg
      601 tgccctccat gtacagcttc atgtgcatgt tctccttaat cagctcgctc attgggccag
      661 gattctcctc cacgtcaccg catgttagga gacttcctct gccctctcct ccggacccgc
      721 cgccggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt
      781 cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct
      841 ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc
      901 tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg
      961 gctccttggg ccggtgcggc gc
//
