LOCUS       sequence-125685-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The RTRG repeat act as a nuclear/cytoplasmic partition signal in the
            expressed fusion protein providing increased nuclear to cytoplasmic 
            partition ratio when increasing the number of repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Christophe-Hobertus C, Christophe D
  TITLE     Identification of a short basic peptide motif able to drive 
            copy-number dependent nuclear accumulation of a linked protein.
  JOURNAL   Protein Pept Lett. 2008;15(4):397-401.
  PUBMED    18473954
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Pept Lett."; date: "2008"; volume: "15(4)"; pages: "397-401"
COMMENT     Sequence Label: pEGFP-RTRG
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(8..112)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      139..594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(226..245)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     436..457
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    601..722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(612..631)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     666..685
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(927..948)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctcaggact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
       61 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      121 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      181 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      241 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      301 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      361 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      421 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      481 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      541 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttacgcgt
      601 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      661 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      721 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      781 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      841 ccggtggatc ccccacgcgt ccgcggacgc tcgagatctg agtacttgta cagctcgtcc
      901 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
      961 tcgttgg
//
