LOCUS       sequence-125691-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The RTRG repeat act as a nuclear/cytoplasmic partition signal in the
            expressed fusion protein providing increased nuclear to cytoplasmic 
            partition ratio when increasing the number of repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Christophe-Hobertus C, Christophe D
  TITLE     Identification of a short basic peptide motif able to drive 
            copy-number dependent nuclear accumulation of a linked protein.
  JOURNAL   Protein Pept Lett. 2008;15(4):397-401.
  PUBMED    18473954
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Pept Lett."; date: "2008"; volume: "15(4)"; pages: "397-401"
COMMENT     Sequence Label: pEGFP-(RTRG)4
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(10..114)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      141..596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(228..247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     438..459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    603..724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(614..633)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     668..687
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(965..986)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgcctcagga ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
       61 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      121 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      481 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      541 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttacgc
      601 gttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
      661 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
      721 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
      781 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      841 atccggtgga tcccccacgc gtccgcccac gcgtccgccc acgcgtccgc ccacgcgtcc
      901 gcggacgctc gagatctgag tacttgtaca gctcgtccat gccgagagtg atcccggcgg
      961 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggg
//
