LOCUS       sequence-125694-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The RTRG repeat act as a nuclear/cytoplasmic partition signal in the
            expressed fusion protein providing increased nuclear to cytoplasmic 
            partition ratio when increasing the number of repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Christophe-Hobertus C, Christophe D
  TITLE     Identification of a short basic peptide motif able to drive 
            copy-number dependent nuclear accumulation of a linked protein.
  JOURNAL   Protein Pept Lett. 2008;15(4):397-401.
  PUBMED    18473954
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Pept Lett."; date: "2008"; volume: "15(4)"; pages: "397-401"
COMMENT     Sequence Label: pEGFP-(RTRG)6
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(11..115)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      142..597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(229..248)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     439..460
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    604..725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(615..634)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     669..688
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
       61 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      121 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      181 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      241 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      301 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      361 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      421 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
      481 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
      541 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttacg
      601 cgttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      661 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      721 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      781 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
      841 gatccggtgg atcccccacg cgtccgccca cgcgtccgcc cacgcgtccg cccacgcgtc
      901 cgcccacgcg tccgcccacg cgtccgcgga cgctcgagat ctgagtactt gtacagctcg
      961 tccatgcc
//
