LOCUS       sequence-125700-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target 
            site in the C. elegans genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD162 (Peft-3::Cas9 + Empty sgRNA)
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(14..337)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(352..378)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(379..399)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aataaagttg tgagactttt ttcttggcgg cacaataaag tttcctcttt ttgttgggtc
       61 actcaacgaa cacaatacaa atatttgagc gctcgcattt ttctcaacct ttcgggttta
      121 aagcacttca aaatggaatt gaagagagat tgtgaaagta catcagacag aatcacattt
      181 tgatagcatt cacttcactc agatgcaaac ttttcagtga ctataaaaag tcataaaata
      241 ataacattaa aaaagcaaat aaattagcga gagaattttt tgacaaaaag aaagaagagt
      301 gatagagaag aagggaatgc ttgaaaggat tttgcattta tcactagttt aggcgtagtc
      361 tgggacgtcg tatgggtaga ccttacgctt cttctttggt ggtccggatc ctccgtctcc
      421 tccgagttgg gagaggtcga tacgggtctc gtagagtccg gtgatggatt ggtggatgag
      481 ggtggcgtcg aggacctcct tggtggaggt gtaacgctta cggtcgatgg tggtgtcgaa
      541 gtacttgaag gcggctgggg ctccgaggtt ggtgagggtg aagaggtgga tgatgttctc
      601 ggcttgctca cggattggct tgtcacggtg cttgttgtag gcggagagga ccttgtcgag
      661 gttggcgtcg gcgaggatga cacgcttgga gaactcggag atttgctcga tgatctcgtc
      721 gaggtagtgc ttgtgttgct cgacgaagag ttgcttttgc tcgttgtcct ctggggatcc
      781 cttgagcttc tcgtagtggg aggcgaggta gaggaagttg acgtacttgg atgggagggc
      841 gagctcgttt cccttttgga gctctccggc ggaggcgagc atacgcttac gtccgttctc
      901 gagctcgaag agggagtact ttgggagctt gatgatgagg tccttcttga cctccttgta
      961 tcccttggcc tc
//
