LOCUS       sequence-125701-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, 
            Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, 
            Thomson JA
  TITLE     Induced pluripotent stem cell lines derived from human somatic 
            cells.
  JOURNAL   Science. 2007 Dec 21. 318(5858):1917-20.
  PUBMED    18029452
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Dec 21. 318(5858):1917-20."
COMMENT     Sequence Label: pGEM-Sox2
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     197..219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     211..228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     212..228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     235..254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        235..253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      284..293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      288..297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
       61 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      121 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      181 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
      241 gactcactat agggcgaatt gggcccgacg tcgcatgctc ccggccgcca tggcggccgc
      301 gggaattcga ttccgcatgt acaacatgat ggagacggag ctgaagccgc cgggcccgca
      361 gcaaacttcg gggggcggcg gcggcaactc caccgcggcg gcggccggcg gcaaccagaa
      421 aaacagcccg gaccgcgtca agcggcccat gaatgccttc atggtgtggt cccgcgggca
      481 gcggcgcaag atggcccagg agaaccccaa gatgcacaac tcggagatca gcaagcgcct
      541 gggcgccgag tggaaacttt tgtcggagac ggagaagcgg ccgttcatcg acgaggctaa
      601 gcggctgcga gcgctgcaca tgaaggagca cccggattat aaataccggc cccggcggaa
      661 aaccaagacg ctcatgaaga aggataagta cacgctgccc ggcgggctgc tggcccccgg
      721 cggcaatagc atggcgagcg gggtcggggt gggcgccggc ctgggcgcgg gcgtgaacca
      781 gcgcatggac agttacgcgc acatgaacgg ctggagcaac ggcagctaca gcatgatgca
      841 ggaccagctg ggctacccgc agcacccggg cctcaatgcg cacggcgcag cgcagatgca
      901 gcccatgcac cgctacgacg tgagcgccct gcagtacaac tccatgacca gctcgcagac
      961 ctacatgaac ggctcgccca cctacagcat gtcctactcg cagcagggca cccctggcat
     1021 ggctcttggc tccatgggtt cggtggtcaa gtccgaggcc agctccagcc cc
//
