LOCUS       sequence-125702-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, 
            Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, 
            Thomson JA
  TITLE     Induced pluripotent stem cell lines derived from human somatic 
            cells.
  JOURNAL   Science. 2007 Dec 21. 318(5858):1917-20.
  PUBMED    18029452
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Dec 21. 318(5858):1917-20."
COMMENT     Sequence Label: pGEM-Sox2
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    350..371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(386..391,392..409,410..416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    424..440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     429..451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     448..464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     448..464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        482..500
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     482..499
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      361 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      421 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      481 tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg agctctccca
      541 tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt tccggccctc acatgtgtga
      601 gaggggcagt gtgccgttaa tggccgtgcc gggcaccggg ccgctctggt agtgctggga
      661 catgtgaagt ctgctggggg cggcgggttc cggcacctcg gcgccgggga gatacatgct
      721 gatcatgtcc cggaggtccc cggcctggca gggcgccctg gagtgggagg aagaggtaac
      781 cacagggggg ctggagctgg cctcggactt gaccaccgaa cccatggagc caagagccat
      841 gccaggggtg ccctgctgcg agtaggacat gctgtaggtg ggcgagccgt tcatgtaggt
      901 ctgcgagctg gtcatggagt tgtactgcag ggcgctcacg tcgtagcggt gcatgggctg
      961 catctgcgct gcgccgtgcg cattgaggcc cgggtgctgc gggtagccca gctggtcctg
     1021 catcatgctg tagctgccgt tgctccagcc gttcatgtgc gcgtaactgt ccatgcgctg
     1081 gttcacgcc
//
