LOCUS       sequence-125720-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd* generation lentiviral packaging plasmid. (*See comments 
            section.) Can be used with 2nd and 3rd generation transfer vectors. 
            Use in conjunction with an envelope plasmid such as pCMV-VSV-G..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pCMV-dR8.2 dvpr
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..58
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
       61 tttcgtcttc aagaattctc atgtttgaca gcttatcatc gccactccag ccagccccac
      121 tcatgttccc cccgcaccac atatgcacat gcacgcatgt acacacaact tgtagtgctc
      181 tttcaaaggt tttattcatt gcagaggggt ggacagggta gtggtgggcc tagttgcagt
      241 agttctccag ttggtagaga gagcagatgc tggtgcagca ctgatccacg atgccgcgct
      301 tctgccgggc cacctccagt gccaaggtct gaaggtcacc ggggcggccg ctcagcagtt
      361 cttgaagtac tccggatgca gctctcgggc catgtgatga aatgctaggc ggctgtcaaa
      421 cctccactct aatacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa
      481 caagcgggtg ttctctcctt cattggcttc ttctaccttc tcttgctcaa ctggtactag
      541 cttgtagcac catccaaagg tcagtggata tctgatccct ggccctggtg tgtagttctg
      601 ccaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttcg
      661 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
      721 agctgccttg taagtcattg gtcttaaagg tacctgaggt gtgactggaa aacccacctc
      781 ctcctcctct tgtgcttcta gccaggcaca atcagcattg gtagctgctg tattgctact
      841 tgtgattgct ccatgttttt ctaggtctcg agatactgct cccaccccat ctgctgctgg
      901 ctcagctcgt ctcattcttt cccttacagt aggccatcca atcacactac tttttgacca
      961 cttgccaccc atcttatagc aaaatccttt ccaagccctg tcttattctt ctaggtatgt
     1021 ggcgaatagc tctacaagct ccttgtacta cttctataac cctatctgtc ccctcagcta
     1081 ctgctatggc tgtggca
//
