LOCUS       sequence-125721-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd* generation lentiviral packaging plasmid. (*See comments 
            section.) Can be used with 2nd and 3rd generation transfer vectors. 
            Use in conjunction with an envelope plasmid such as pCMV-VSV-G..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pCMV-dR8.2 dvpr
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
      121 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc
      181 ttggcttctt atgcgacgga tcgatcccgt aataagcttc gaggtccgcg gccgcgttga
      241 cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag agcgtcagta
      301 ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaaa
      361 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
      421 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
      481 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
      541 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
      601 caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca cagcaatcag
      661 gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca tcaggccata
      721 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa
      781 gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt aaacaccatg
      841 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag
      901 gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc accaggccag
      961 atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca ggaacaaata
     1021 ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag atggataatc
     1081 ct
//
