LOCUS       sequence-125724-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
       61 acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattc gaattcaagg
      121 atcaacaagt ttgtacaaaa aagcaggctg gcgccaccat ggacaagaag tactccattg
      181 ggctcgatat cggtaccaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc
      241 cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca
      301 ttggagccct cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag
      361 cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta
      421 atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg
      481 aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt
      541 accatgaaaa gtacccaacc atatatcatc tgaggaagaa gctggtagac agtactgata
      601 aggctgactt gcggttgatc tatctcgcgc tggcgcacat gatcaaattt cggggacact
      661 tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac
      721 tggttcagac ttacaatcag cttttcgagg agaacccgat caacgcatcc ggcgttgacg
      781 ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac
      841 agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc
      901 tgacccc
//
