LOCUS       sequence-125727-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral, selectable fluorescent GFP tagging of nuclei..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Gersbach
  TITLE     TMG reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FU-H2B-GFP-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             140..517
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      530..539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(581..602)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
       61 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca
      121 ggtcgactct agagccacca tgccagagcc agcgaagtct gctcccgccc cgaaaaaggg
      181 ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca agcgcagccg
      241 caaggagagc tattccatct atgtgtacaa ggttctgaag caggtccacc ctgacaccgg
      301 catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt tcgagcgcat
      361 cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc tcgaccatca cctccaggga
      421 gatccagacg gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg ccgtgtccga
      481 gggtactaag gccatcacca agtacaccag cgctaaggat ccaccggtcg ccaccatggt
      541 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      601 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      661 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      721 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      781 cgacttc
//
