LOCUS       sequence-125729-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(408..434)
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             complement(447..536)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(537..557)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgtaatacga ctcactatag ttctagaggc tcgagtcatt acccggggtc tttctgacgg
       61 aggtggatct tggtgtgctg tgtcagggtg ctccggtccg cgaacttcct gccgcagatg
      121 tcacaggcga agggcttttc tcctgtgtgg gtgcggatgt gccgtgtcag gtggtgggca
      181 cagctgaagt tcctcatgca gatgcggcat tgaaacggtt tttgccctgt gtgaatgcgg
      241 atgtgccgtg tcaggtggct cttctcactg aagcggcggt cacaggactc gacaggacac
      301 gcgtatggct tctcaccagt gtgcgtacgt tggtgccgtg tcaggtctcc gctctgcgag
      361 aaggacttgc cacattcagg acatttatag ggctttccgg attcgaaatc cagtttcttt
      421 ttcttagctg ccggagcggc ggatccagcg taatctggaa cgtcataagg atacgatcct
      481 gcatagtccg ggacgtcata gggatagccc gcatagtcag gaacatcgta tgggtatacc
      541 ttcctcttct tcttgggccc gccggtgccg tctccaccga gctgagagag gtcgattctt
      601 gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac ctcctttgta
      661 gaggtgtact tctttctgtc tatggtggtg tcgaagtact tgaaggctgc aggcgcgccc
      721 aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat gggcttatcc
      781 ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag gatcactctt
      841 ttggagaact cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg ttgttccacg
      901 aacagctgct tctgctcatt atcttcggga gacc
//
