LOCUS       sequence-125739-        4130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human AKT3 [NM_005465.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4130)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E006 Hs.AKT3-nostop
FEATURES             Location/Qualifiers
     source          1..4130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1621..1720)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2097..2888
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2981..3569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3470..3489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3723..3740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3899..3926)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4018..4104)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgag cgatgttacc attgtgaaag aaggttgggt tcagaagagg ggagaatata
      241 taaaaaactg gaggccaaga tacttccttt tgaagacaga tggctcattc ataggatata
      301 aagagaaacc tcaagatgtg gatttacctt atcccctcaa caacttttca gtggcaaaat
      361 gccagttaat gaaaacagaa cgaccaaagc caaacacatt tataatcaga tgtctccagt
      421 ggactactgt tatagagaga acatttcatg tagatactcc agaggaaagg gaagaatgga
      481 cagaagctat ccaggctgta gcagacagac tgcagaggca agaagaggag agaatgaatt
      541 gtagtccaac ttcacaaatt gataatatag gagaggaaga gatggatgcc tctacaaccc
      601 atcataaaag aaagacaatg aatgattttg actatttgaa actactaggt aaaggcactt
      661 ttgggaaagt tattttggtt cgagagaagg caagtggaaa atactatgct atgaagattc
      721 tgaagaaaga agtcattatt gcaaaggatg aagtggcaca cactctaact gaaagcagag
      781 tattaaagaa cactagacat ccctttttaa catccttgaa atattccttc cagacaaaag
      841 accgtttgtg ttttgtgatg gaatatgtta atgggggcga gctgtttttc catttgtcga
      901 gagagcgggt gttctctgag gaccgcacac gtttctatgg tgcagaaatt gtctctgcct
      961 tggactatct acattccgga aagattgtgt accgtgatct caagttggag aatctaatgc
     1021 tggacaaaga tggccacata aaaattacag attttggact ttgcaaagaa gggatcacag
     1081 atgcagccac catgaagaca ttctgtggca ctccagaata tctggcacca gaggtgttag
     1141 aagataatga ctatggccga gcagtagact ggtggggcct aggggttgtc atgtatgaaa
     1201 tgatgtgtgg gaggttacct ttctacaacc aggaccatga gaaacttttt gaattaatat
     1261 taatggaaga cattaaattt cctcgaacac tctcttcaga tgcaaaatca ttgctttcag
     1321 ggctcttgat aaaggatcca aataaacgcc ttggtggagg accagatgat gcaaaagaaa
     1381 ttatgagaca cagtttcttc tctggagtaa actggcaaga tgtatatgat aaaaagcttg
     1441 tacctccttt taaacctcaa gtaacatctg agacagatac tagatatttt gatgaagaat
     1501 ttacagctca gactattaca ataacaccac ctgaaaaata tgatgaggat ggtatggact
     1561 gcatggacaa tgagaggcgg ccgcatttcc ctcaattttc ctactctgca agtggacgag
     1621 aatcaacttt cttgtacaaa gttggcatta taggaaagca ttgcttatca atttgttgca
     1681 acgaacaggt cactatcagt caaaataaaa tcattatttg catgcaagct tagatcctct
     1741 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     1801 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1861 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1921 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     1981 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     2041 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     2101 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     2161 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     2221 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     2281 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     2341 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     2401 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     2461 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     2521 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     2581 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     2641 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2701 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     2761 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     2821 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     2881 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3121 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3361 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3721 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3781 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3841 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     3901 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     3961 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     4021 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     4081 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
//
