LOCUS       sequence-125741-        7553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human ALK [NM_004304.4] without stop codon (for
            C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7553)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E008 Hs.ALK-nostop
FEATURES             Location/Qualifiers
     source          1..7553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5044..5143)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5520..6311
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6404..6992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6893..6912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7146..7163
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7322..7349)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(7441..7527)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggg agccatcggg ctcctgtggc tcctgccgct gctgctttcc acggcagctg
      241 tgggctccgg gatggggacc ggccagcgcg cgggctcccc agctgcgggg ccgccgctgc
      301 agccccggga gccactcagc tactcgcgcc tgcagaggaa gagtctggca gttgacttcg
      361 tggtgccctc gctcttccgt gtctacgccc gggacctact gctgccacca tcctcctcgg
      421 agctgaaggc tggcaggccc gaggcccgcg gctcgctagc tctggactgc gccccgctgc
      481 tcaggttgct ggggccggcg ccgggggtct cctggaccgc cggttcacca gccccggcag
      541 aggcccggac gctgtccagg gtgctgaagg gcggctccgt gcgcaagctc cggcgtgcca
      601 agcagttggt gctggagctg ggcgaggagg cgatcttgga gggttgcgtc gggccccccg
      661 gggaggcggc tgtggggctg ctccagttca atctcagcga gctgttcagt tggtggattc
      721 gccaaggcga agggcgactg aggatccgcc tgatgcccga gaagaaggcg tcggaagtgg
      781 gcagagaggg aaggctgtcc gcggcaattc gcgcctccca gccccgcctt ctcttccaga
      841 tcttcgggac tggtcatagc tccttggaat caccaacaaa catgccttct ccttctcctg
      901 attattttac atggaatctc acctggataa tgaaagactc cttccctttc ctgtctcatc
      961 gcagccgata tggtctggag tgcagctttg acttcccctg tgagctggag tattcccctc
     1021 cactgcatga cctcaggaac cagagctggt cctggcgccg catcccctcc gaggaggcct
     1081 cccagatgga cttgctggat gggcctgggg cagagcgttc taaggagatg cccagaggct
     1141 cctttctcct tctcaacacc tcagctgact ccaagcacac catcctgagt ccgtggatga
     1201 ggagcagcag tgagcactgc acactggccg tctcggtgca caggcacctg cagccctctg
     1261 gaaggtacat tgcccagctg ctgccccaca acgaggctgc aagagagatc ctcctgatgc
     1321 ccactccagg gaagcatggt tggacagtgc tccagggaag aatcgggcgt ccagacaacc
     1381 catttcgagt ggccctggaa tacatctcca gtggaaaccg cagcttgtct gcagtggact
     1441 tctttgccct gaagaactgc agtgaaggaa catccccagg ctccaagatg gccctgcaga
     1501 gctccttcac ttgttggaat gggacagtcc tccagcttgg gcaggcctgt gacttccacc
     1561 aggactgtgc ccagggagaa gatgagagcc agatgtgccg gaaactgcct gtgggttttt
     1621 actgcaactt tgaagatggc ttctgtggct ggacccaagg cacactgtca ccccacactc
     1681 ctcaatggca ggtcaggacc ctaaaggatg cccggttcca ggaccaccaa gaccatgctc
     1741 tattgctcag taccactgat gtccccgctt ctgaaagtgc tacagtgacc agtgctacgt
     1801 ttcctgcacc gatcaagagc tctccatgtg agctccgaat gtcctggctc attcgtggag
     1861 tcttgagggg aaacgtgtcc ttggtgctag tggagaacaa aaccgggaag gagcaaggca
     1921 ggatggtctg gcatgtcgcc gcctatgaag gcttgagcct gtggcagtgg atggtgttgc
     1981 ctctcctcga tgtgtctgac aggttctggc tgcagatggt cgcatggtgg ggacaaggat
     2041 ccagagccat cgtggctttt gacaatatct ccatcagcct ggactgctac ctcaccatta
     2101 gcggagagga caagatcctg cagaatacag cacccaaatc aagaaacctg tttgagagaa
     2161 acccaaacaa ggagctgaaa cccggggaaa attcaccaag acagaccccc atctttgacc
     2221 ctacagttca ttggctgttc accacatgtg gggccagcgg gccccatggc cccacccagg
     2281 cacagtgcaa caacgcctac cagaactcca acctgagcgt ggaggtgggg agcgagggcc
     2341 ccctgaaagg catccagatc tggaaggtgc cagccaccga cacctacagc atctcgggct
     2401 acggagctgc tggcgggaaa ggcgggaaga acaccatgat gcggtcccac ggcgtgtctg
     2461 tgctgggcat cttcaacctg gagaaggatg acatgctgta catcctggtt gggcagcagg
     2521 gagaggacgc ctgccccagt acaaaccagt taatccagaa agtctgcatt ggagagaaca
     2581 atgtgataga agaagaaatc cgtgtgaaca gaagcgtgca tgagtgggca ggaggcggag
     2641 gaggaggggg tggagccacc tacgtattta agatgaagga tggagtgccg gtgcccctga
     2701 tcattgcagc cggaggtggt ggcagggcct acggggccaa gacagacacg ttccacccag
     2761 agagactgga gaataactcc tcggttctag ggctaaacgg caattccgga gccgcaggtg
     2821 gtggaggtgg ctggaatgat aacacttcct tgctctgggc cggaaaatct ttgcaggagg
     2881 gtgccaccgg aggacattcc tgcccccagg ccatgaagaa gtgggggtgg gagacaagag
     2941 ggggtttcgg agggggtgga ggggggtgct cctcaggtgg aggaggcgga ggatatatag
     3001 gcggcaatgc agcctcaaac aatgaccccg aaatggatgg ggaagatggg gtttccttca
     3061 tcagtccact gggcatcctg tacaccccag ctttaaaagt gatggaaggc cacggggaag
     3121 tgaatattaa gcattatcta aactgcagtc actgtgaggt agacgaatgt cacatggacc
     3181 ctgaaagcca caaggtcatc tgcttctgtg accacgggac ggtgctggct gaggatggcg
     3241 tctcctgcat tgtgtcaccc accccggagc cacacctgcc actctcgctg atcctctctg
     3301 tggtgacctc tgccctcgtg gccgccctgg tcctggcttt ctccggcatc atgattgtgt
     3361 accgccggaa gcaccaggag ctgcaagcca tgcagatgga gctgcagagc cctgagtaca
     3421 agctgagcaa gctccgcacc tcgaccatca tgaccgacta caaccccaac tactgctttg
     3481 ctggcaagac ctcctccatc agtgacctga aggaggtgcc gcggaaaaac atcaccctca
     3541 ttcggggtct gggccatggc gcctttgggg aggtgtatga aggccaggtg tccggaatgc
     3601 ccaacgaccc aagccccctg caagtggctg tgaagacgct gcctgaagtg tgctctgaac
     3661 aggacgaact ggatttcctc atggaagccc tgatcatcag caaattcaac caccagaaca
     3721 ttgttcgctg cattggggtg agcctgcaat ccctgccccg gttcatcctg ctggagctca
     3781 tggcgggggg agacctcaag tccttcctcc gagagacccg ccctcgcccg agccagccct
     3841 cctccctggc catgctggac cttctgcacg tggctcggga cattgcctgt ggctgtcagt
     3901 atttggagga aaaccacttc atccaccgag acattgctgc cagaaactgc ctcttgacct
     3961 gtccaggccc tggaagagtg gccaagattg gagacttcgg gatggcccga gacatctaca
     4021 gggcgagcta ctatagaaag ggaggctgtg ccatgctgcc agttaagtgg atgcccccag
     4081 aggccttcat ggaaggaata ttcacttcta aaacagacac atggtccttt ggagtgctgc
     4141 tatgggaaat cttttctctt ggatatatgc cataccccag caaaagcaac caggaagttc
     4201 tggagtttgt caccagtgga ggccggatgg acccacccaa gaactgccct gggcctgtat
     4261 accggataat gactcagtgc tggcaacatc agcctgaaga caggcccaac tttgccatca
     4321 ttttggagag gattgaatac tgcacccagg acccggatgt aatcaacacc gctttgccga
     4381 tagaatatgg tccacttgtg gaagaggaag agaaagtgcc tgtgaggccc aaggaccctg
     4441 agggggttcc tcctctcctg gtctctcaac aggcaaaacg ggaggaggag cgcagcccag
     4501 ctgccccacc acctctgcct accacctcct ctggcaaggc tgcaaagaaa cccacagctg
     4561 cagagatctc tgttcgagtc cctagagggc cggccgtgga agggggacac gtgaatatgg
     4621 cattctctca gtccaaccct ccttcggagt tgcacaaggt ccacggatcc agaaacaagc
     4681 ccaccagctt gtggaaccca acgtacggct cctggtttac agagaaaccc accaaaaaga
     4741 ataatcctat agcaaagaag gagccacacg acaggggtaa cctggggctg gagggaagct
     4801 gtactgtccc acctaacgtt gcaactggga gacttccggg ggcctcactg ctcctagagc
     4861 cctcttcgct gactgccaat atgaaggagg tacctctgtt caggctacgt cacttccctt
     4921 gtgggaatgt caattacggc taccagcaac agggcttgcc cttagaagcc gctactgccc
     4981 ctggagctgg tcattacgag gataccattc tgaaaagcaa gaatagcatg aaccagcctg
     5041 ggccctcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt
     5101 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc
     5161 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     5221 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     5281 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     5341 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     5401 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     5461 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     5521 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     5581 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     5641 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     5701 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     5761 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     5821 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     5881 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     5941 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     6001 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     6061 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     6121 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     6181 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     6241 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     6301 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     6361 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6421 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6481 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6541 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6601 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6781 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7021 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7081 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7141 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7201 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7261 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     7321 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     7381 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     7441 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     7501 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg
//
