LOCUS       sequence-125742-        4523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human ARAF [NM_001256196.1] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4523)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E009 Hs.ARAF
FEATURES             Location/Qualifiers
     source          1..4523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2014..2113)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2490..3281
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3374..3962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3863..3882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4116..4133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4292..4319)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4411..4497)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gccaccacgg ggcccccctg ccaatggggc cgagccatcc cgggcagtgg
      241 gcaccgtcaa agtatacctg cccaacaagc aacgcacggt ggtgactgtc cgggatggca
      301 tgagtgtcta cgactctcta gacaaggccc tgaaggtgcg gggtctaaat caggactgct
      361 gtgtggtcta ccgactcatc aagggacgaa agacggtcac tgcctgggac acagccattg
      421 ctcccctgga tggcgaggag ctcattgtcg aggtccttga agatgtcccg ctgaccatgc
      481 acaattttgt acggaagacc ttcttcagcc tggcgttctg tgacttctgc cttaagtttc
      541 tgttccatgg cttccgttgc caaacctgtg gctacaagtt ccaccagcat tgttcctcca
      601 aggtccccac agtctgtgtt gacatgagta ccaaccgcca acagccctca aggttctacc
      661 acagtgtcca ggatttgtcc ggaggctcca gacagcatga ggctccctcg aaccgccccc
      721 tgaatgagtt gctaaccccc cagggtccca gcccccgcac ccagcactgt gacccggagc
      781 acttcccctt ccctgcccca gccaatgccc ccctacagcg catccgctcc acgtccactc
      841 ccaacgtcca tatggtcagc accacggccc ccatggactc caacctcatc cagctcactg
      901 gccagagttt cagcactgat gctgccggta gtagaggagg tagtgatgga accccccggg
      961 ggagccccag cccagccagc gtgtcctcgg ggaggaagtc cccacattcc aagtcaccag
     1021 cagagcagcg cgagcggaag tccttggccg atgacaagaa gaaagtgaag aacctggggt
     1081 accgggactc aggctattac tgggaggtac cacccagtga ggtgcagctg ctgaagagga
     1141 tcgggacggg ctcgtttggc accgtgtttc gagggcggtg gcatggcgat gtggccgtga
     1201 aggtgctcaa ggtgtcccag cccacagctg agcaggccca ggctttcaag aatgagatgc
     1261 aggtgctcag gaagacgcga catgtcaaca tcttgctgtt tatgggcttc atgacccggc
     1321 cgggatttgc catcatcaca cagtggtgtg agggctccag cctctaccat cacctgcatg
     1381 tggccgacac acgcttcgac atggtccagc tcatcgacgt ggcccggcag actgcccagg
     1441 gcatggacta cctccatgcc aagaacatca tccaccgaga tctcaagtct aacaacatct
     1501 tcctacatga ggggctcacg gtgaagatcg gtgactttgg cttggccaca gtgaagactc
     1561 gatggagcgg ggcccagccc ttggagcagc cctcaggatc tgtgctgtgg atggcagctg
     1621 aggtgatccg tatgcaggac ccgaacccct acagcttcca gtcagacgtc tatgcctacg
     1681 gggttgtgct ctacgagctt atgactggct cactgcctta cagccacatt ggctgccgtg
     1741 accagattat ctttatggtg ggccgtggct atctgtcccc ggacctcagc aaaatctcca
     1801 gcaactgccc caaggccatg cggcgcctgc tgtctgactg cctcaagttc cagcgggagg
     1861 agcggcccct cttcccccag atcctggcca caattgagct gctgcaacgg tcactcccca
     1921 agattgagcg gagtgcctcg gaaccctcct tgcaccgcac ccaggccgat gagttgcctg
     1981 cctgcctact cagcgcagcc cgccttgtgc cttaatcaac tttcttgtac aaagttggca
     2041 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2101 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     2161 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     2221 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     2281 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     2341 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2401 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2461 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     2521 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2581 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     2641 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2701 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2761 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2821 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2881 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2941 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     3001 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     3061 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     3121 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     3181 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     3241 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     3301 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     3361 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3421 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3481 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3541 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3601 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3661 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3721 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     3781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4141 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4201 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     4261 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     4321 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     4381 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     4441 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     4501 cctggcagtt ccctactctc gcg
//
