LOCUS       sequence-125743-        4520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human ARAF [NM_001256196.1] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4520)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E010 Hs.ARAF-nostop
FEATURES             Location/Qualifiers
     source          1..4520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2011..2110)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2487..3278
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3371..3959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3860..3879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4113..4130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4289..4316)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4408..4494)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gccaccacgg ggcccccctg ccaatggggc cgagccatcc cgggcagtgg
      241 gcaccgtcaa agtatacctg cccaacaagc aacgcacggt ggtgactgtc cgggatggca
      301 tgagtgtcta cgactctcta gacaaggccc tgaaggtgcg gggtctaaat caggactgct
      361 gtgtggtcta ccgactcatc aagggacgaa agacggtcac tgcctgggac acagccattg
      421 ctcccctgga tggcgaggag ctcattgtcg aggtccttga agatgtcccg ctgaccatgc
      481 acaattttgt acggaagacc ttcttcagcc tggcgttctg tgacttctgc cttaagtttc
      541 tgttccatgg cttccgttgc caaacctgtg gctacaagtt ccaccagcat tgttcctcca
      601 aggtccccac agtctgtgtt gacatgagta ccaaccgcca acagccctca aggttctacc
      661 acagtgtcca ggatttgtcc ggaggctcca gacagcatga ggctccctcg aaccgccccc
      721 tgaatgagtt gctaaccccc cagggtccca gcccccgcac ccagcactgt gacccggagc
      781 acttcccctt ccctgcccca gccaatgccc ccctacagcg catccgctcc acgtccactc
      841 ccaacgtcca tatggtcagc accacggccc ccatggactc caacctcatc cagctcactg
      901 gccagagttt cagcactgat gctgccggta gtagaggagg tagtgatgga accccccggg
      961 ggagccccag cccagccagc gtgtcctcgg ggaggaagtc cccacattcc aagtcaccag
     1021 cagagcagcg cgagcggaag tccttggccg atgacaagaa gaaagtgaag aacctggggt
     1081 accgggactc aggctattac tgggaggtac cacccagtga ggtgcagctg ctgaagagga
     1141 tcgggacggg ctcgtttggc accgtgtttc gagggcggtg gcatggcgat gtggccgtga
     1201 aggtgctcaa ggtgtcccag cccacagctg agcaggccca ggctttcaag aatgagatgc
     1261 aggtgctcag gaagacgcga catgtcaaca tcttgctgtt tatgggcttc atgacccggc
     1321 cgggatttgc catcatcaca cagtggtgtg agggctccag cctctaccat cacctgcatg
     1381 tggccgacac acgcttcgac atggtccagc tcatcgacgt ggcccggcag actgcccagg
     1441 gcatggacta cctccatgcc aagaacatca tccaccgaga tctcaagtct aacaacatct
     1501 tcctacatga ggggctcacg gtgaagatcg gtgactttgg cttggccaca gtgaagactc
     1561 gatggagcgg ggcccagccc ttggagcagc cctcaggatc tgtgctgtgg atggcagctg
     1621 aggtgatccg tatgcaggac ccgaacccct acagcttcca gtcagacgtc tatgcctacg
     1681 gggttgtgct ctacgagctt atgactggct cactgcctta cagccacatt ggctgccgtg
     1741 accagattat ctttatggtg ggccgtggct atctgtcccc ggacctcagc aaaatctcca
     1801 gcaactgccc caaggccatg cggcgcctgc tgtctgactg cctcaagttc cagcgggagg
     1861 agcggcccct cttcccccag atcctggcca caattgagct gctgcaacgg tcactcccca
     1921 agattgagcg gagtgcctcg gaaccctcct tgcaccgcac ccaggccgat gagttgcctg
     1981 cctgcctact cagcgcagcc cgccttgtgc cttcaacttt cttgtacaaa gttggcatta
     2041 taggaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2101 tcattatttg catgcaagct tagatcctct agaccagcca ggacagaaat gcctcgactt
     2161 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     2221 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2281 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2341 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2401 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2461 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2521 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2581 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2641 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2701 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2761 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2821 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2881 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2941 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3001 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3061 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3121 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3181 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3241 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3301 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3361 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3421 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3481 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3541 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3601 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3661 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3721 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     3781 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3841 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3901 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3961 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4021 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4081 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4141 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4201 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4261 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4321 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4381 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4441 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     4501 ggcagttccc tactctcgcg
//
