LOCUS       sequence-125764-        3896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human CYTH2 [NM_017457.5] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E031 Hs.CYTH2
FEATURES             Location/Qualifiers
     source          1..3896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1387..1486)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1863..2654
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2747..3335
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3236..3255
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3489..3506
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3665..3692)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3784..3870)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga ggacggcgtc tatgaacccc cagacctgac tccggaggag cggatggagc
      241 tggagaacat ccggcggcgg aagcaggagc tgctggtgga gattcagcgc ctgcgggagg
      301 agctcagtga agccatgagc gaggtggagg ggctggaggc caatgagggc agtaagacct
      361 tgcaacggaa ccggaagatg gcaatgggca ggaagaagtt caacatggac cccaagaagg
      421 ggatccagtt cttggtggag aatgaactgc tgcagaacac acccgaggag atcgcccgct
      481 tcctgtacaa gggcgagggg ctgaacaaga cagccatcgg ggactacctg ggggagaggg
      541 aagaactgaa cctggcagtg ctccatgctt ttgtggatct gcatgagttc accgacctca
      601 atctggtgca ggccctcagg cagtttctat ggagctttcg cctacccgga gaggcccaga
      661 aaattgaccg gatgatggag gccttcgccc agcgatactg cctgtgcaac cctggggttt
      721 tccagtccac agacacgtgc tatgtgctgt ccttcgccgt catcatgctc aacaccagtc
      781 tccacaatcc caatgtccgg gacaagccgg gcctggagcg ctttgtggcc atgaaccggg
      841 gcatcaacga gggcggggac ctgcctgagg agctgctcag gaacctgtac gacagcatcc
      901 gaaatgagcc cttcaagatt cctgaggatg acgggaatga cctgacccac accttcttca
      961 acccggaccg ggagggctgg ctcctgaagc tgggaggggg ccgggtgaag acgtggaagc
     1021 ggcgctggtt tatcctcaca gacaactgcc tctactactt tgagtacacc acggacaagg
     1081 agccccgagg aatcatcccc ctggagaatc tgagcatccg agaggtggac gacccccgga
     1141 aaccgaactg ctttgaactt tacatcccca acaacaaggg gcagctcatc aaagcctgca
     1201 aaactgaggc ggacggccga gtggtggagg gaaaccacat ggtgtaccgg atctcggccc
     1261 ccacgcagga ggagaaggac gagtggatca agtccatcca ggcggctgtg agtgtggacc
     1321 ccttctatga gatgctggca gcgagaaaga agcggatttc agtcaagaag aagcaggagc
     1381 agccctaatc aactttcttg tacaaagttg gcattatagg aaagcattgc ttatcaattt
     1441 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg caagcttaga
     1501 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1561 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1621 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1681 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1741 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1801 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1861 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1921 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1981 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2041 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2101 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2161 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2221 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2281 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2341 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2401 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2461 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2521 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2581 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2641 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     2701 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2761 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2821 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2881 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2941 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3001 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3061 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3121 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3181 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3241 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3301 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3361 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3421 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3481 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3541 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3601 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3661 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3721 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3781 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3841 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
