LOCUS       sequence-125765-        3893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human CYTH2 [NM_017457.5] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3893)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E032 Hs.CYTH2-nostop
FEATURES             Location/Qualifiers
     source          1..3893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1384..1483)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1860..2651
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2744..3332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3233..3252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3486..3503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3662..3689)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3781..3867)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga ggacggcgtc tatgaacccc cagacctgac tccggaggag cggatggagc
      241 tggagaacat ccggcggcgg aagcaggagc tgctggtgga gattcagcgc ctgcgggagg
      301 agctcagtga agccatgagc gaggtggagg ggctggaggc caatgagggc agtaagacct
      361 tgcaacggaa ccggaagatg gcaatgggca ggaagaagtt caacatggac cccaagaagg
      421 ggatccagtt cttggtggag aatgaactgc tgcagaacac acccgaggag atcgcccgct
      481 tcctgtacaa gggcgagggg ctgaacaaga cagccatcgg ggactacctg ggggagaggg
      541 aagaactgaa cctggcagtg ctccatgctt ttgtggatct gcatgagttc accgacctca
      601 atctggtgca ggccctcagg cagtttctat ggagctttcg cctacccgga gaggcccaga
      661 aaattgaccg gatgatggag gccttcgccc agcgatactg cctgtgcaac cctggggttt
      721 tccagtccac agacacgtgc tatgtgctgt ccttcgccgt catcatgctc aacaccagtc
      781 tccacaatcc caatgtccgg gacaagccgg gcctggagcg ctttgtggcc atgaaccggg
      841 gcatcaacga gggcggggac ctgcctgagg agctgctcag gaacctgtac gacagcatcc
      901 gaaatgagcc cttcaagatt cctgaggatg acgggaatga cctgacccac accttcttca
      961 acccggaccg ggagggctgg ctcctgaagc tgggaggggg ccgggtgaag acgtggaagc
     1021 ggcgctggtt tatcctcaca gacaactgcc tctactactt tgagtacacc acggacaagg
     1081 agccccgagg aatcatcccc ctggagaatc tgagcatccg agaggtggac gacccccgga
     1141 aaccgaactg ctttgaactt tacatcccca acaacaaggg gcagctcatc aaagcctgca
     1201 aaactgaggc ggacggccga gtggtggagg gaaaccacat ggtgtaccgg atctcggccc
     1261 ccacgcagga ggagaaggac gagtggatca agtccatcca ggcggctgtg agtgtggacc
     1321 ccttctatga gatgctggca gcgagaaaga agcggatttc agtcaagaag aagcaggagc
     1381 agccctcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt
     1441 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc
     1501 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     1561 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     1621 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     1681 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     1741 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     1801 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     1861 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1921 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1981 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     2041 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     2101 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     2161 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     2221 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     2281 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     2341 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     2401 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     2461 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     2521 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     2581 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     2641 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     2701 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2761 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2821 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2881 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2941 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3001 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3061 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3121 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3181 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3241 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3301 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3361 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3421 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3481 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3541 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3601 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     3661 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     3721 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     3781 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     3841 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg
//
