LOCUS       sequence-125766-        3797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human DUSP1 [NM_004417.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3797)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E033 Hs.DUSP1
FEATURES             Location/Qualifiers
     source          1..3797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1288..1387)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1764..2555
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2648..3236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3137..3156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3390..3407
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3566..3593)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3685..3771)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggt catggaagtg ggcaccctgg acgctggagg cctgcgggcg ctgctggggg
      241 agcgagcggc gcaatgcctg ctgctggact gccgctcctt cttcgctttc aacgccggcc
      301 acatcgccgg ctctgtcaac gtgcgcttca gcaccatcgt gcggcgccgg gccaagggcg
      361 ccatgggcct ggagcacatc gtgcccaacg ccgagctccg cggccgcctg ctggccggcg
      421 cctaccacgc cgtggtgttg ctggacgagc gcagcgccgc cctggacggc gccaagcgcg
      481 acggcaccct ggccctggcg gccggcgcgc tctgccgcga ggcgcgcgcc gcgcaagtct
      541 tcttcctcaa aggaggatac gaagcgtttt cggcttcctg cccggagctg tgcagcaaac
      601 agtcgacccc catggggctc agccttcccc tgagtactag cgtccctgac agcgcggaat
      661 ctgggtgcag ttcctgcagt accccactct acgatcaggg tggcccggtg gaaatcctgc
      721 cctttctgta cctgggcagt gcgtatcacg cttcccgcaa ggacatgctg gatgccttgg
      781 gcataactgc cttgatcaac gtctcagcca attgtcccaa ccattttgag ggtcactacc
      841 agtacaagag catccctgtg gaggacaacc acaaggcaga catcagctcc tggttcaacg
      901 aggccattga cttcatagac tccatcaaga atgctggagg aagggtgttt gtccactgcc
      961 aggcaggcat ttcccggtca gccaccatct gccttgctta ccttatgagg actaatcgag
     1021 tcaagctgga cgaggccttt gagtttgtga agcagaggcg aagcatcatc tctcccaact
     1081 tcagcttcat gggccagctg ctgcagtttg agtcccaggt gctggctccg cactgttcgg
     1141 cagaggctgg gagccccgcc atggctgtgc tcgaccgagg cacctccacc accaccgtgt
     1201 tcaacttccc cgtctccatc cctgtccact ccacgaacag tgcgctgagc taccttcaga
     1261 gccccattac gacctctccc agctgctaat caactttctt gtacaaagtt ggcattatag
     1321 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1381 ttatttgcat gcaagcttag atcctctaga ccagccagga cagaaatgcc tcgacttcgc
     1441 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1501 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1561 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1621 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1681 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1741 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     1801 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1861 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     1921 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1981 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2041 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2101 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2161 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2221 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2281 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2341 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2401 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2461 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2521 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     2581 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2641 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2701 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2761 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2821 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2881 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2941 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3001 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     3061 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3121 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3181 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3241 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3301 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3361 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3421 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3481 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     3541 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     3601 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     3661 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     3721 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     3781 agttccctac tctcgcg
//
