LOCUS       sequence-125767-        3794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human DUSP1 [NM_004417.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3794)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E034 Hs.DUSP1-nostop
FEATURES             Location/Qualifiers
     source          1..3794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1285..1384)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1761..2552
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2645..3233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3134..3153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3387..3404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3563..3590)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3682..3768)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggt catggaagtg ggcaccctgg acgctggagg cctgcgggcg ctgctggggg
      241 agcgagcggc gcaatgcctg ctgctggact gccgctcctt cttcgctttc aacgccggcc
      301 acatcgccgg ctctgtcaac gtgcgcttca gcaccatcgt gcggcgccgg gccaagggcg
      361 ccatgggcct ggagcacatc gtgcccaacg ccgagctccg cggccgcctg ctggccggcg
      421 cctaccacgc cgtggtgttg ctggacgagc gcagcgccgc cctggacggc gccaagcgcg
      481 acggcaccct ggccctggcg gccggcgcgc tctgccgcga ggcgcgcgcc gcgcaagtct
      541 tcttcctcaa aggaggatac gaagcgtttt cggcttcctg cccggagctg tgcagcaaac
      601 agtcgacccc catggggctc agccttcccc tgagtactag cgtccctgac agcgcggaat
      661 ctgggtgcag ttcctgcagt accccactct acgatcaggg tggcccggtg gaaatcctgc
      721 cctttctgta cctgggcagt gcgtatcacg cttcccgcaa ggacatgctg gatgccttgg
      781 gcataactgc cttgatcaac gtctcagcca attgtcccaa ccattttgag ggtcactacc
      841 agtacaagag catccctgtg gaggacaacc acaaggcaga catcagctcc tggttcaacg
      901 aggccattga cttcatagac tccatcaaga atgctggagg aagggtgttt gtccactgcc
      961 aggcaggcat ttcccggtca gccaccatct gccttgctta ccttatgagg actaatcgag
     1021 tcaagctgga cgaggccttt gagtttgtga agcagaggcg aagcatcatc tctcccaact
     1081 tcagcttcat gggccagctg ctgcagtttg agtcccaggt gctggctccg cactgttcgg
     1141 cagaggctgg gagccccgcc atggctgtgc tcgaccgagg cacctccacc accaccgtgt
     1201 tcaacttccc cgtctccatc cctgtccact ccacgaacag tgcgctgagc taccttcaga
     1261 gccccattac gacctctccc agctgctcaa ctttcttgta caaagttggc attataggaa
     1321 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1381 tttgcatgca agcttagatc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     1441 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     1501 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1561 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     1621 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1681 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     1741 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     1801 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     1861 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     1921 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     1981 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2041 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2101 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2161 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2221 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2281 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2341 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2401 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2461 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2521 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     2581 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2761 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3001 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     3061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     3541 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     3601 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     3661 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     3721 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     3781 tccctactct cgcg
//
