LOCUS       sequence-125770-        3251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human DUSP3 [NM_004090.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3251)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E037 Hs.DUSP3
FEATURES             Location/Qualifiers
     source          1..3251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(742..841)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1218..2009
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2102..2690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2591..2610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2844..2861
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3020..3047)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3139..3225)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtc gggctcgttc gagctctcgg tgcaggatct caacgacctg ctctcggacg
      241 gcagcggctg ctacagcctc ccgagccagc cctgcaacga ggtcaccccg cggatctacg
      301 tgggcaacgc gtctgtggct caggacatcc ccaagctgca gaaactaggc atcacccatg
      361 tgctgaacgc ggctgagggc aggtccttca tgcacgtcaa caccaatgcc aacttctaca
      421 aggactccgg catcacatac ctgggcatca aggccaacga cacacaggag ttcaacctca
      481 gcgcttactt tgaaagggct gccgacttca ttgaccaggc tttggctcaa aagaatggcc
      541 gggtgctcgt ccactgccgg gaaggttata gccgctcccc aacgctagtt atcgcctacc
      601 tcatgatgcg gcagaagatg gacgtcaagt ctgccctgag catcgtgagg cagaaccgtg
      661 agatcggccc caacgatggc ttcctggccc agctctgcca gctcaatgac agactagcca
      721 aggaggggaa gttgaaaccc taatcaactt tcttgtacaa agttggcatt ataggaaagc
      781 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
      841 gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
      901 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
      961 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     1021 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1081 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1141 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1201 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1261 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1321 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     1381 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1441 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1501 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     1561 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     1621 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     1681 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     1741 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     1801 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     1861 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     1921 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     1981 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     2041 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2101 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2161 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2221 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2281 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2341 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2401 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2461 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     2521 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2581 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2641 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2701 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2761 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2821 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2881 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2941 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3001 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3061 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3121 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3181 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3241 ctactctcgc g
//
