LOCUS       sequence-125774-        3848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human DUSP5 [NM_004419.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3848)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E041 Hs.DUSP5
FEATURES             Location/Qualifiers
     source          1..3848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1339..1438)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1815..2606
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2699..3287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3188..3207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3441..3458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3617..3644)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3736..3822)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgaa ggtcacgtcg ctcgacgggc gccagctgcg caagatgctc cgcaaggagg
      241 cggcggcgcg ctgcgtggtg ctcgactgcc ggccctatct ggccttcgct gcctcgaacg
      301 tgcgcggctc gctcaacgtc aacctcaact cggtggtgct gcggcgggcc cggggcggcg
      361 cggtgtcggc gcgctacgtg ctgcccgacg aggcggcgcg cgcgcggctc ctgcaggagg
      421 gcggcggcgg cgtcgcggcc gtggtggtgc tggaccaggg cagccgccac tggcagaagc
      481 tgcgagagga gagcgccgcg cgtgtcgtcc tcacctcgct actcgcttgc ctacccgccg
      541 gcccgcgggt ctacttcctc aaagggggat atgagacttt ctactcggaa tatcctgagt
      601 gttgcgtgga tgtaaaaccc atttcacaag agaagattga gagtgagaga gccctcatca
      661 gccagtgtgg aaaaccagtg gtaaatgtca gctacaggcc agcttatgac cagggtggcc
      721 cagttgaaat ccttcccttc ctctaccttg gaagtgccta ccatgcatcc aagtgcgagt
      781 tcctcgccaa cttgcacatc acagccctgc tgaatgtctc ccgacggacc tccgaggcct
      841 gcatgaccca cctacactac aaatggatcc ctgtggaaga cagccacacg gctgacatta
      901 gctcccactt tcaagaagca atagacttca ttgactgtgt cagggaaaag ggaggcaagg
      961 tcctggtcca ctgtgaggct gggatctccc gttcacccac catctgcatg gcttacctta
     1021 tgaagaccaa gcagttccgc ctgaaggagg ccttcgatta catcaagcag aggaggagca
     1081 tggtctcgcc caactttggc ttcatgggcc agctcctgca gtacgaatct gagatcctgc
     1141 cctccacgcc caacccccag cctccctcct gccaagggga ggcagcaggc tcttcactga
     1201 taggccattt gcagacactg agccctgaca tgcagggtgc ctactgcaca ttccctgcct
     1261 cggtgctggc accggtgcct acccactcaa cagtctcaga gctcagcaga agccctgtgg
     1321 caacggccac atcctgctaa tcaactttct tgtacaaagt tggcattata ggaaagcatt
     1381 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     1441 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1501 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     1561 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     1621 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1681 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1741 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1801 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1861 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1921 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     1981 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2041 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2101 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2161 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2221 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2281 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2341 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2401 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2461 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2521 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     2581 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     2641 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2701 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2761 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2821 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     2881 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2941 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3001 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3061 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     3121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3541 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3601 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     3661 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     3721 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3781 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     3841 ctctcgcg
//
