LOCUS       sequence-125776-        3839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human DUSP6 [NM_001946.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3839)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E043 Hs.DUSP6
FEATURES             Location/Qualifiers
     source          1..3839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1330..1429)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1806..2597
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2690..3278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3179..3198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3432..3449
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3608..3635)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3727..3813)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgat agatacgctc agacccgtgc ccttcgcgtc ggaaatggcg atcagcaaga
      241 cggtggcgtg gctcaacgag cagctggagc tgggcaacga gcggctgctg ctgatggact
      301 gccggccgca ggagctatac gagtcgtcgc acatcgagtc ggccatcaac gtggccatcc
      361 cgggcatcat gctgcggcgc ctgcagaagg gtaacctgcc ggtgcgcgcg ctcttcacgc
      421 gcggcgagga ccgggaccgc ttcacccggc gctgtggcac cgacacagtg gtgctctacg
      481 acgagagcag cagcgactgg aacgagaata cgggcggcga gtcggtgctc gggctgctgc
      541 tcaagaagct caaggacgag ggctgccggg cgttctacct ggaaggtggc ttcagtaagt
      601 tccaagccga gttctccctg cattgcgaga ccaatctaga cggctcgtgt agcagcagct
      661 cgccgccgtt gccagtgctg gggctcgggg gcctgcggat cagctctgac tcttcctcgg
      721 acatcgagtc tgaccttgac cgagacccca atagtgcaac agactcggat ggtagtccgc
      781 tgtccaacag ccagccttcc ttcccagtgg agatcttgcc cttcctctac ttgggctgtg
      841 ccaaagactc caccaacttg gacgtgttgg aggaattcgg catcaagtac atcttgaacg
      901 tcacccccaa tttgccgaat ctctttgaga acgcaggaga gtttaaatac aagcaaatcc
      961 ccatctcgga tcactggagc caaaacctgt cccagttttt ccctgaggcc atttctttca
     1021 tagatgaagc ccggggcaag aactgtggtg tcttggtaca ttgcttggct ggcattagcc
     1081 gctcagtcac tgtgactgtg gcttacctta tgcagaagct caatctgtcg atgaacgatg
     1141 cctatgacat tgtcaaaatg aaaaaatcca acatatcccc taacttcaac ttcatgggtc
     1201 agctgctgga cttcgagagg acgctgggac tcagcagccc atgtgacaac agggttccag
     1261 cacagcagct gtattttacc accccttcca accagaatgt ataccaggtg gactctctgc
     1321 aatctacgta atcaactttc ttgtacaaag ttggcattat aggaaagcat tgcttatcaa
     1381 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc atgcaagctt
     1441 agatcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1501 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1561 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1621 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1681 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1741 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1801 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1861 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1921 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     1981 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2041 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2101 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2161 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2221 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2281 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2341 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2401 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2461 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2521 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2581 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2641 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2821 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3061 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3301 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3361 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3421 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3481 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3541 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3601 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3661 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3721 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3781 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcg
//
