LOCUS       sequence-125777-        3836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human DUSP6 [NM_001946.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3836)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E044 Hs.DUSP6-nostop
FEATURES             Location/Qualifiers
     source          1..3836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1327..1426)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1803..2594
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2687..3275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3176..3195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3429..3446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3605..3632)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3724..3810)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgat agatacgctc agacccgtgc ccttcgcgtc ggaaatggcg atcagcaaga
      241 cggtggcgtg gctcaacgag cagctggagc tgggcaacga gcggctgctg ctgatggact
      301 gccggccgca ggagctatac gagtcgtcgc acatcgagtc ggccatcaac gtggccatcc
      361 cgggcatcat gctgcggcgc ctgcagaagg gtaacctgcc ggtgcgcgcg ctcttcacgc
      421 gcggcgagga ccgggaccgc ttcacccggc gctgtggcac cgacacagtg gtgctctacg
      481 acgagagcag cagcgactgg aacgagaata cgggcggcga gtcggtgctc gggctgctgc
      541 tcaagaagct caaggacgag ggctgccggg cgttctacct ggaaggtggc ttcagtaagt
      601 tccaagccga gttctccctg cattgcgaga ccaatctaga cggctcgtgt agcagcagct
      661 cgccgccgtt gccagtgctg gggctcgggg gcctgcggat cagctctgac tcttcctcgg
      721 acatcgagtc tgaccttgac cgagacccca atagtgcaac agactcggat ggtagtccgc
      781 tgtccaacag ccagccttcc ttcccagtgg agatcttgcc cttcctctac ttgggctgtg
      841 ccaaagactc caccaacttg gacgtgttgg aggaattcgg catcaagtac atcttgaacg
      901 tcacccccaa tttgccgaat ctctttgaga acgcaggaga gtttaaatac aagcaaatcc
      961 ccatctcgga tcactggagc caaaacctgt cccagttttt ccctgaggcc atttctttca
     1021 tagatgaagc ccggggcaag aactgtggtg tcttggtaca ttgcttggct ggcattagcc
     1081 gctcagtcac tgtgactgtg gcttacctta tgcagaagct caatctgtcg atgaacgatg
     1141 cctatgacat tgtcaaaatg aaaaaatcca acatatcccc taacttcaac ttcatgggtc
     1201 agctgctgga cttcgagagg acgctgggac tcagcagccc atgtgacaac agggttccag
     1261 cacagcagct gtattttacc accccttcca accagaatgt ataccaggtg gactctctgc
     1321 aatctacgtc aactttcttg tacaaagttg gcattatagg aaagcattgc ttatcaattt
     1381 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg caagcttaga
     1441 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1501 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1561 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1621 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1681 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1741 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1801 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1861 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1921 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     1981 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2041 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2101 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2161 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2221 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2281 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2341 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2401 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2461 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2521 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2581 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     2641 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2701 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2761 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2821 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2881 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2941 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3001 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3061 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3121 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3181 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3241 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3301 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3361 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3421 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3481 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3541 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3601 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3661 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3721 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3781 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
