LOCUS       sequence-125778-        4007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human E2F1 [NM_005225.2] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4007)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E045 Hs.E2F1
FEATURES             Location/Qualifiers
     source          1..4007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1498..1597)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1974..2765
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2858..3446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3347..3366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3600..3617
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3776..3803)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3895..3981)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc cttggccggg gcccctgcgg gcggcccatg cgcgccggcg ctggaggccc
      241 tgctcggggc cggcgcgctg cggctgctcg actcctcgca gatcgtcatc atctccgccg
      301 cgcaggacgc cagcgccccg ccggctccca ccggccccgc ggcgcccgcc gccggcccct
      361 gcgaccctga cctgctgctc ttcgccacac cgcaggcgcc ccggcccaca cccagtgcgc
      421 cgcggcccgc gctcggccgc ccgccggtga agcggaggct ggacctggaa actgaccatc
      481 agtacctggc cgagagcagt gggccagctc ggggcagagg ccgccatcca ggaaaaggtg
      541 tgaaatcccc gggggagaag tcacgctatg agacctcact gaatctgacc accaagcgct
      601 tcctggagct gctgagccac tcggctgacg gtgtcgtcga cctgaactgg gctgccgagg
      661 tgctgaaggt gcagaagcgg cgcatctatg acatcaccaa cgtccttgag ggcatccagc
      721 tcattgccaa gaagtccaag aaccacatcc agtggctggg cagccacacc acagtgggcg
      781 tcggcggacg gcttgagggg ttgacccagg acctccgaca gctgcaggag agcgagcagc
      841 agctggacca cctgatgaat atctgtacta cgcagctgcg cctgctctcc gaggacactg
      901 acagccagcg cctggcctac gtgacgtgtc aggaccttcg tagcattgca gaccctgcag
      961 agcagatggt tatggtgatc aaagcccctc ctgagaccca gctccaagcc gtggactctt
     1021 cggagaactt tcagatctcc cttaagagca aacaaggccc gatcgatgtt ttcctgtgcc
     1081 ctgaggagac cgtaggtggg atcagccctg ggaagacccc atcccaggag gtcacttctg
     1141 aggaggagaa cagggccact gactctgcca ccatagtgtc accaccacca tcatctcccc
     1201 cctcatccct caccacagat cccagccagt ctctactcag cctggagcaa gaaccgctgt
     1261 tgtcccggat gggcagcctg cgggctcccg tggacgagga ccgcctgtcc ccgctggtgg
     1321 cggccgactc gctcctggag catgtgcggg aggacttctc cggcctcctc cctgaggagt
     1381 tcatcagcct ttccccaccc cacgaggccc tcgactacca cttcggcctc gaggagggcg
     1441 agggcatcag agacctcttc gactgtgact ttggggacct cacccccctg gatttctaat
     1501 caactttctt gtacaaagtt ggcattatag gaaagcattg cttatcaatt tgttgcaacg
     1561 aacaggtcac tatcagtcaa aataaaatca ttatttgcat gcaagcttag atcctctaga
     1621 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     1681 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     1741 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1801 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     1861 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1921 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     1981 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2041 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2101 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2161 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2221 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2281 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2341 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2401 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2461 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2521 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     2581 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     2641 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2701 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     2761 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2821 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2881 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2941 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3001 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3061 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3121 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3181 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3241 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3301 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3361 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3421 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3481 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3541 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3601 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3661 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3721 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3781 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3841 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3901 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3961 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcg
//
