LOCUS       sequence-125779-        4004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human E2F1 [NM_005225.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4004)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E046 Hs.E2F1-nostop
FEATURES             Location/Qualifiers
     source          1..4004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1495..1594)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1971..2762
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2855..3443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3344..3363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3597..3614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3773..3800)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3892..3978)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc cttggccggg gcccctgcgg gcggcccatg cgcgccggcg ctggaggccc
      241 tgctcggggc cggcgcgctg cggctgctcg actcctcgca gatcgtcatc atctccgccg
      301 cgcaggacgc cagcgccccg ccggctccca ccggccccgc ggcgcccgcc gccggcccct
      361 gcgaccctga cctgctgctc ttcgccacac cgcaggcgcc ccggcccaca cccagtgcgc
      421 cgcggcccgc gctcggccgc ccgccggtga agcggaggct ggacctggaa actgaccatc
      481 agtacctggc cgagagcagt gggccagctc ggggcagagg ccgccatcca ggaaaaggtg
      541 tgaaatcccc gggggagaag tcacgctatg agacctcact gaatctgacc accaagcgct
      601 tcctggagct gctgagccac tcggctgacg gtgtcgtcga cctgaactgg gctgccgagg
      661 tgctgaaggt gcagaagcgg cgcatctatg acatcaccaa cgtccttgag ggcatccagc
      721 tcattgccaa gaagtccaag aaccacatcc agtggctggg cagccacacc acagtgggcg
      781 tcggcggacg gcttgagggg ttgacccagg acctccgaca gctgcaggag agcgagcagc
      841 agctggacca cctgatgaat atctgtacta cgcagctgcg cctgctctcc gaggacactg
      901 acagccagcg cctggcctac gtgacgtgtc aggaccttcg tagcattgca gaccctgcag
      961 agcagatggt tatggtgatc aaagcccctc ctgagaccca gctccaagcc gtggactctt
     1021 cggagaactt tcagatctcc cttaagagca aacaaggccc gatcgatgtt ttcctgtgcc
     1081 ctgaggagac cgtaggtggg atcagccctg ggaagacccc atcccaggag gtcacttctg
     1141 aggaggagaa cagggccact gactctgcca ccatagtgtc accaccacca tcatctcccc
     1201 cctcatccct caccacagat cccagccagt ctctactcag cctggagcaa gaaccgctgt
     1261 tgtcccggat gggcagcctg cgggctcccg tggacgagga ccgcctgtcc ccgctggtgg
     1321 cggccgactc gctcctggag catgtgcggg aggacttctc cggcctcctc cctgaggagt
     1381 tcatcagcct ttccccaccc cacgaggccc tcgactacca cttcggcctc gaggagggcg
     1441 agggcatcag agacctcttc gactgtgact ttggggacct cacccccctg gatttctcaa
     1501 ctttcttgta caaagttggc attataggaa agcattgctt atcaatttgt tgcaacgaac
     1561 aggtcactat cagtcaaaat aaaatcatta tttgcatgca agcttagatc ctctagacca
     1621 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1681 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1741 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1801 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     1861 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     1921 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     1981 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2041 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2101 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2161 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2221 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2281 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2341 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2401 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2461 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2521 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2581 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2641 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2701 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2761 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2821 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2881 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2941 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3001 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3061 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3121 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3241 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3361 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3421 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3481 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3541 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3601 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3661 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3721 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3781 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3841 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     3901 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     3961 agcctttcgt tttatttgat gcctggcagt tccctactct cgcg
//
