LOCUS       sequence-125781-        4004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human E2F2 [NM_004091.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4004)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E048 Hs.E2F2-nostop
FEATURES             Location/Qualifiers
     source          1..4004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1495..1594)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1971..2762
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2855..3443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3344..3363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3597..3614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3773..3800)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3892..3978)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgct gcaagggccc cgggccttgg cttcggccgc tgggcagacc ccgaaggtgg
      241 tgcccgcgat gagccccaca gagctgtggc catccggcct cagcagcccc cagctctgcc
      301 cagctactgc tacctactac acaccgctgt acccgcagac ggcgcctccc gcagcggcgc
      361 caggcacctg cctcgacgcc actccccacg gacccgaggg ccaagttgtg cgatgcctgc
      421 cggcaggccg gctgccggcc aaaaggaagc tggatctgga ggggattggg aggcccgtcg
      481 tccctgagtt cccaaccccc aaggggaagt gcatcagagt ggatggcctc cccagcccca
      541 aaacccccaa atcccccggg gagaagactc ggtatgacac ttcgctgggg ctgctcacca
      601 agaagttcat ttacctcctg agcgagtcag aggatggggt cctggacctg aactgggccg
      661 ctgaggtgct ggacgtgcag aagcggcgca tctatgacat caccaacgtg ctggaaggca
      721 tccagctcat ccgcaagaag gccaagaaca acatccagtg ggtaggcagg ggaatgtttg
      781 aagaccccac cagacctggg aagcagcaac agctggggca ggagctgaag gagctgatga
      841 acacggagca ggccttggac catctcatcc agagctgctc tctgagcttc aagcacctga
      901 ctgaggacaa ggccaacaag aggctggcct atgtgactta ccaggatatc cgtgctgttg
      961 gcaactttaa ggagcagaca gtgattgccg tcaaggcccc tccgcagacg agactggaag
     1021 tgcccgacag gactgaggac aacctgcaga tatatctcaa gagcacccaa gggcccatcg
     1081 aagtctacct gtgcccagag gaggtgcagg agccggacag tccttccgag gagcctctcc
     1141 cctctacctc caccctctgc cccagccctg actctgccca gcccagcagc agcaccgacc
     1201 ctagcatcat ggagcccaca gcatcctcag tgccagcacc agcgccaacc ccccagcagg
     1261 ccccaccgcc tccatccctg gtccccttgg aggctactga cagcctgctg gagctgccgc
     1321 acccactcct gcagcagact gaggaccagt tcctgtcccc gaccctggcg tgcagctccc
     1381 ctctgatcag cttctcccca tccttggacc aggacgacta cctgtggggc ttggaggcgg
     1441 gtgagggcat cagcgatctc ttcgactcct acgaccttgg ggacctgttg attaattcaa
     1501 ctttcttgta caaagttggc attataggaa agcattgctt atcaatttgt tgcaacgaac
     1561 aggtcactat cagtcaaaat aaaatcatta tttgcatgca agcttagatc ctctagacca
     1621 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1681 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1741 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1801 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     1861 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     1921 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     1981 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2041 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2101 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2161 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2221 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2281 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2341 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2401 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2461 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2521 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2581 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2641 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2701 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2761 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2821 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2881 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2941 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3001 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3061 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3121 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3241 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3361 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3421 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3481 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3541 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3601 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3661 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3721 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3781 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3841 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     3901 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     3961 agcctttcgt tttatttgat gcctggcagt tccctactct cgcg
//
