LOCUS       sequence-125790-        3056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human EIF4EBP2 [NM_004096.4] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3056)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E057 Hs.EIF4EBP2
FEATURES             Location/Qualifiers
     source          1..3056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(547..646)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1023..1814
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1907..2495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2396..2415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2649..2666
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2825..2852)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2944..3030)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtc ctcgtcagcc ggcagcggcc accagcccag ccagagccgc gccatcccca
      241 cccgcaccgt ggccatcagc gacgccgcgc agctacctca tgactattgc accacgcccg
      301 gggggacgct cttctccacc acaccgggag gaactcgaat catttatgac agaaagtttc
      361 tgttggatcg tcgcaattct cccatggctc agaccccacc ctgccacctg cccaatatcc
      421 caggagtcac tagccctggc accttaattg aagactccaa agtagaagta aacaatttga
      481 acaacttgaa caatcacgac aggaaacatg cagttgggga tgatgctcag ttcgagatgg
      541 acatctaatc aactttcttg tacaaagttg gcattatagg aaagcattgc ttatcaattt
      601 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg caagcttaga
      661 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
      721 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
      781 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
      841 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
      901 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
      961 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1021 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1081 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1141 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     1201 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     1261 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     1321 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     1381 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     1441 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     1501 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     1561 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     1621 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     1681 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     1741 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     1801 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     1861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2041 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2281 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2581 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2641 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2701 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2761 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     2821 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     2881 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     2941 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3001 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
