LOCUS       sequence-125796-        4019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human ETS1 [NM_005238.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4019)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E063 Hs.ETS1
FEATURES             Location/Qualifiers
     source          1..4019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1510..1609)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1986..2777
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2870..3458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3359..3378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3612..3629
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3788..3815)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3907..3993)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgaa ggcggccgtc gatctcaagc cgactctcac catcatcaag acggaaaaag
      241 tcgatctgga gcttttcccc tccccggata tggaatgtgc agatgtccca ctattaactc
      301 caagcagcaa agaaatgatg tctcaagcat taaaagctac tttcagtggt ttcactaaag
      361 aacagcaacg actggggatc ccaaaagacc cccggcagtg gacagaaacc catgttcggg
      421 actgggtgat gtgggctgtg aatgaattca gcctgaaagg tgtagacttc cagaagttct
      481 gtatgaatgg agcagccctc tgcgccctgg gtaaagactg ctttctcgag ctggccccag
      541 actttgttgg ggacatctta tgggaacatc tagagatcct gcagaaagag gatgtgaaac
      601 catatcaagt taatggagtc aacccagcct atccagaatc ccgctatacc tcggattact
      661 tcattagcta tggtattgag catgcccagt gtgttccacc atcggagttc tcagagccca
      721 gcttcatcac agagtcctat cagacgctcc atcccatcag ctcggaagag ctcctctccc
      781 tcaagtatga gaatgactac ccctcggtca ttctccgaga ccctctccag acagacacct
      841 tgcagaatga ctactttgct atcaaacaag aagtcgtcac cccagacaac atgtgcatgg
      901 ggaggaccag tcgtggtaaa ctcgggggcc aggactcttt tgaaagcata gagagctacg
      961 atagttgtga tcgcctcacc cagtcctgga gcagccagtc atctttcaac agcctgcagc
     1021 gtgttccctc ctatgacagc ttcgactcag aggactatcc ggctgccctg cccaaccaca
     1081 agcccaaggg caccttcaag gactatgtgc gggaccgtgc tgacctcaat aaggacaagc
     1141 ctgtcattcc tgctgctgcc ctagctggct acacaggcag tggaccaatc cagctatggc
     1201 agtttcttct ggaattactc actgataaat cctgtcagtc ttttatcagc tggacaggag
     1261 atggctggga attcaaactt tctgacccag atgaggtggc caggagatgg ggaaagagga
     1321 aaaacaaacc taagatgaat tatgagaaac tgagccgtgg cctacgctac tattacgaca
     1381 aaaacatcat ccacaagaca gcggggaaac gctacgtgta ccgctttgtg tgtgacctgc
     1441 agagcctgct ggggtacacc cctgaggagc tgcacgccat gctggacgtc aagccagatg
     1501 ccgacgagta atcaactttc ttgtacaaag ttggcattat aggaaagcat tgcttatcaa
     1561 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc atgcaagctt
     1621 agatcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1681 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1741 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1801 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1861 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1921 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1981 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2041 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2101 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2161 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2221 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2281 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2341 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2401 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2461 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2521 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2581 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2641 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2701 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2761 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2821 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3001 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3241 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3481 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3541 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3601 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3661 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3721 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3781 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3841 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3901 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3961 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcg
//
